SARS-CoV-2 Spike RBD (N501Y) Protein (C-His)

Target Name: SARS-CoV2 RBD, Spike RBD Protein, RBD Protein

Source: SARS-CoV-2 S protein (accession number QHD43416.1) RBD (Arg319-Phe541, N501Y) with C-terminus 8xHis tag is expressed in CHO cells.

Molecular Weight:
The 234 amino acid protein has the predicted molecular weight of 26.4 kDa. The protein migrates at approximately 35-40 kDa on SDS-PAGE with DTT-reduced condition.

Purity: >95% based on SDS-PAGE

Formulation: PBS, 0.22 µm filtered

Vial Size: 25µg, 100µg

Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.

Storage and Handling:
Quick spin the vial after receiving. Unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered PBS. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier protein such as FBS and BSA need to be added if further dilution is needed.