SEAP Reporter Gene Assay Kit

Secreted Alkaline Phosphatase (SEAP) Reporter Gene is a really easy, sensitive and fast assay that utilizes enzyme activity of alkaline phosphatase to dephosphorylate the chemiluminescent alkaline phosphatase substrate CSPD into an unstable dioxetane anion which decomposes and emits light.

SEAP encodes a truncated form of the placental enzyme that lacks the membrane anchoring domain, thereby allowing the protein to secrete efficiently from transfected cells.

Changes in levels of SEAP activity detected in the culture medium are directly proportional to changes in intracellular concentrations of SEAP mRNA and protein.

Advantages & Features
- Convenient: a single set of cells is used for both the SEAP assay and another purpose.
- Time-saving protocol: results in 55 minutes due the elimination of cell lysate preparation.
- Cost-effective: allows performance directly in a microtiter plate.
- Sensitive: assayed even in low cell concentrations.
- Secreted from transfected cells into the culture medium.

Includes
- 3 x 96 W Solid Plate (white)
- 3 units of lid
- 50 μL Alkaline Phosphatase Standard
- 15 mL SEAP Substrate (Luminescence)

Applications
Measurement the levels of SEAP in the culture medium of transfected cells.

Shipping & Storage
Shipping: Ambient Temperature.
Storage: 4 ºC, except Component solution containing CSPD® Substrate (2-6 °C), Placental Alkaline Phosphatase Positive Control (-20 °C).
Recommendations: Protect SEAP Substrate from light.