Sema-3A & NRP1/Plexin A1 Receptor Immunocytochemistry Kit

Background:
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

Background References
Kolodkin, A.L. et al. (1993) Cell 75:1389.
Luo, Y. et al. (1993) Cell 75:217.
He, Z. & Tessier-Lavigne, M. (1997) Cell 90:739.
Takahashi, T. et al. (1999) Cell 99:59.
Tamagnone, L. et al. (1999) Cell 99:71.
Takahashi, T. & Strittmatter, S.M. (2001) Neuron 29:429.
Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.

Buffer/Storage:
Rabbit polyclonal antibodies and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

Product References:
NP2111 Boro, A. et al. (2015) Am J Transl Res. 7(3):640-653 WB: human osteosarcoma cell lines
PP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. WB: rat brain
NP2111 Ohkusu-Tsukada, K. et al. (2010) Int J Cancer. Epub 15 MAR. ELISA: sNRP1 mouse serum
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord