Senescence Detection Kit (SA-β-gal Staining)

Senescence Detection Kit is a Fast, Convenient & Easy-to-use kit that measures activity of SA-B-Gal in cells cultures by hydrolysis of X-gal, which results in the accumulation of a distinctive blue color in senescent cells.

Senescence cells display a phenotype like increase of cell size, distinctive flat morphology, changes in gene expression and activity of senescence-associate ß-galactosidase (SA-ß-gal).

It represents tumor suppressor mechanism for this reason cellular senescence has become an increasingly target in the development of novel therapeutics.

Advantages & Features
- Fast, convenient and easy procedure: takes 28 minutes to results with minimal handling steps.
- The specific histochemical marker is only present in senescent cells and is not found in pre-senescent, quiescent or immortal cells

Includes
- 150 mg X-Gal (lyophilized)
- 60 mL PBS (10x)
- 15 mL Fixative Solution (10x)
- 15 mL Staining Solution (5x)
- 1 x 1,5 mL Staining Solution Supplement A (100x)
- 1 x 1,5 mL Staining Solution Supplement B (100x)


Applications
Histochemically detect SA-β-Galactosidase activity in cultured cell and tissue sections.

Shipping & Storage
Shipped in: Gel Pack.
Storage:
- X-Gal (150 mg, Lyophilized) at -20°C, protected from light.
- 10X Fixative Solution, 10X Staining Solution and 100X Staining Supplement (A and B) can be stored at +4°C. If precipitation occurs, simply warm up the solution to 37°C to solubilize the precipitates.
- Store the 10x PBS solution at Room Temperature or 4ºC.