Separopore® 2B
€0.00
In stock
SKU
BW-20181045
Application:
Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good separation over a wide molecular weight range. Larger volumes are available at discounted prices (enquire).
Technical Specifications:
Matrix: Separopore® 2B (agarose beads, 2%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as slurry in 20% ethanol
Chemical stability: Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride
Physical stability: Negligible volume variation due to changes in pH or ionic strength
Sterilization: Chemical
References:
Inhibition of fibrinogen binding to human platelets by S-nitroso-N-acetylcysteine. J Biol Chem. (1990) 265: 19028-34.
Nitric oxide released from activated platelets inhibits platelet recruitment. J Clin Invest. (1997) 100: 350-6.
Identification of the fibrinogen receptor on human platelets by photoaffinity labeling. J Biol Chem. (1982) 257: 8049-54.
An experimental model of pulmonary arterial granulomatous inflammation. Am J Pathol. (1973) 71: 365-72.
Identification and characterization of LAMP-1 as an activation-dependent platelet surface glycoprotein. J Biol Chem. (1990) 265: 18531-7.
Modulation of the arrestin-clathrin interaction in cells. Characterization of beta-arrestin dominant-negative mutants. J Biol Chem. (1997) 272: 32507-12.
Identification of lysosome-associated membrane protein-2 as an activation-dependent platelet surface glycoprotein. Blood. (1992) 80: 1470-5.
Inhibition of platelet adhesion to surfaces of extracorporeal circuits by disintegrins. RGD-containing peptides from viper venoms. Circulation. (1990) 82: 261-73.
Studies of the mechanism of tunicamycin in hibition of IgA and IgE secretion by plasma cells. J Biol Chem. (1977) 252: 4402-8.
Characterization of the binding of human tissue-type plasminogen activator to platelets. J Biol Chem. (1989) 264: 15869-74.
The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein. Proc Natl Acad Sci U S A. (1991) 88: 11212-6.
Lipoprotein(a) binds to human platelets and attenuates plasminogen binding and activation. Biochemistry. (1993) 32: 4628-33.
Regulation of glycoprotein IIB/IIIA exposure on platelets stimulated with alpha-thrombin. Blood. (1992) 79: 82-90.
Ultrastructural localization of human platelet thrombospondin, fibrinogen, fibronectin, and von Willebrand factor in frozen thin section. Blood. (1985) 65: 929-38.
Association of fibrin with the platelet cytoskeleton. J Biol Chem. (1984) 259: 5247-54.
Immunochemical analysis of normal and mutant forms of human pyruvate dehydrogenase. Biochem J. (1986) 239: 89-96.
Stimulation of mitogen-activated protein kinase and Na+/H+ exchanger in human platelets. Differential effect of phorbol ester and vasopressin. J Biol Chem. (1996) 271: 16494-9.
Tissue plasminogen activator promotes platelet disaggregation in plasma. J Clin Invest. (1987) 79: 1749-55.
Synthesis of cartilage-specific proteoglycan by suspension cultures of adult chondrocytes. Biochem J. (1977) 164: 269-72.
Identification of gallbladder mucin-bilirubin complex in human cholesterol gallstone matrix. Effects of reducing agents on in vitro dissolution of matrix and intact gallstones. J Clin Invest. (1985) 76: 439-45.
A role for prostaglandins and thromboxanes in the exposure of platelet fibrinogen receptors. J Clin Invest. (1981) 68: 981-7.
Protein kinase C and cyclic AMP regulate reversible exposure of binding sites for fibrinogen on the glycoprotein IIB-IIIA complex of human platelets. Biochem J. (1991) 273: 115-20.
Solid lipid nanocarriers as drug carriers II. Plasma stability and biodistribution of solid lipid nanoparticles containing the lipophilic prodrug 3'-azido-3'-deoxythymidine palmitate in mice. Int. J. Pharm. (1998) 174: 71-80.
The effect of molecular weight on heparin binding to platelets. Br J Haematol. (1990) 74: 306-12.
A study of the kinetics of ADP-triggered platelet shape change. Blood. (1984) 64: 896-906.
Plasma protein adsorption patterns on liposomes: establishment of analytical procedure. Electrophoresis. (1996) 17: 607-11.
Arrestin/clathrin interaction. Localization of the clathrin binding domain of nonvisual arrestins to the carboxy terminus. J Biol Chem. (1997) 272: 15011-6.
Characterization of recombinant and brain neuropsin, a plasticity-related serine protease. J Biol Chem. (1998) 273: 11189-96.
Immunologic and neuropharmacologic stimulation of mucous glycoprotein release from human airways in vitro. J Clin Invest. (1980) 66: 1400-8.
Multimeric structure of platelet factor VIII/von Willebrand factor: the presence of larger multimers and their reassociation with thrombin-stimulated platelets. Blood. (1982) 60: 1132-8.
Variations in the composition of bovine hip articular cartilage with distance from the articular surface. Biochem J. (1981) 195: 535-43.
Analysis of CD9, CD32 and p67 signalling: use of degranulated platelets indicates direct involvement of CD9 and p67 in integrin activation. Br J Haematol. (1997) 96: 275-86.
Platelet stimulation releases a cAMP-dependent protein kinase that specifically phosphorylates a plasma protein. Proc Natl Acad Sci U S A. (1988) 85: 7541-5.
Heparin binding to resting and activated platelets. Blood. (1989) 74: 238-43.
Hydrogen peroxide is involved in collagen-induced platelet activation. Blood. (1998) 91: 484-90.
Stimulation of platelet activation and aggregation by a carboxyl-terminal peptide from thrombospondin binding to the integrin-associated protein receptor. J Biol Chem. (1997) 272: 1323-30.
Activation of soluble guanylate cyclase from rat lung by incubation or by hydrogen peroxide. J Biol Chem. (1976) 251: 7304-12.
Purification and properties of a kinase which phosphorylates and inactivates acetyl-CoA carboxylase. J Biol Chem. (1982) 257: 1897-901.
Association of coagulation factor V with the platelet cytoskeleton. J Biol Chem. (1982) 257: 4557-63.
Protein kinase C mediates translocation of type II phosphatidylinositol 5-phosphate 4-kinase required for platelet alpha-granule secretion. J Biol Chem. (2003) 278: 8126-34.
Cyclopiazonic acid and thapsigargin induce platelet aggregation resulting from Ca2+ influx through Ca2+ store-activated Ca2+-channels. Eur J Pharmacol. (1998) 341: 343-7.
Cation gradient dependence of the steps in thrombin stimulation of human platelets. J Biol Chem. (1984) 259: 1502-8.
Regulation of rat liver acetyl-CoA carboxylase: Regulation of phosphorylation and inactivation of acetyl-CoA carboxylase by the adenylate energy charge. J Biol Chem. (1980) 255: 2308-14.
Ultrastructural localization of coagulation factor V in human platelets. Blood. (1986) 68: 244-9.
Detection of soluble class I molecules (non HLA-A or HLA-B) in serum, spleen membranes and lymphocytes in culture. Eur J Immunol. (1989) 19: 1835-9.
Immunolocalization of beta 1 integrins in platelets and platelet-derived microvesicles. Blood. (1993) 82: 1197-203.
Coenzyme A activation of acetyl-CoA carboxylase. J Biol Chem. (1981) 256: 2289-96.
The actin cytoskeleton differentially regulates platelet alpha-granule and dense-granule secretion. Blood. (2005) 105: 3879-87.
The platelet cytoskeleton contains elements of the prothrombinase complex. J Biol Chem. (1984) 259: 6947-51.
Comparison of carbohydrate-containing substances from non-calcified and calcified portions of bovine costal cartilage. J Biochem. (1979) 85: 1067-73.
Continuous binding of the PAF molecule to its receptor is necessary for the long-term aggregation of platelets. Am J Physiol. (1998) 274: C47-57.
Biochemical isolation of a membrane microdomain from resting platelets highly enriched in the plasma membrane glycoprotein CD36. Biochem J. (1996) 319: 67-72.
Labeling of human platelet plasma membrane thromboxane A2/prostaglandin H2 receptors using SQB, a novel biotinylated receptor probe. Biochem Pharmacol. (1996) 52: 763-70.
Localization of dipeptidyl aminopeptidase yscIV in the plasma membrane of Saccharomyces cerevisiae. J Gen Microbiol. (1990) 136: 419-23.
Staphylococcus aureus alpha-toxin attack on human platelets promotes assembly of the prothrombinase complex. J Biol Chem. (1990) 265: 14377-81.
Evidence for inhibitors of the cell surface protease guanidinobenzoatase. J Enzyme Inhib. (1986) 1: 127-37.
Interaction of T lymphocytes and macrophages with cultured vascular endothelial cells: attachment, invasion, and subsequent degradation of the subendothelial extracellular matrix. J Cell Physiol. (1984) 118: 169-78.
Assembly of proteoglycan aggregates in cultures of chondrocytes from bovine tracheal cartilage. Biochem J. (1981) 199: 17-29.
Rat liver microsomal and lysosomal beta-glucuronidases differ in both carbohydrate and amino acid compositions. Proc Natl Acad Sci U S A. (1978) 75: 3080-4.
Pattern of endogenous lectins in a human epithelial tumor. Cancer Res. (1985) 45: 253-7.
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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®
Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good separation over a wide molecular weight range. Larger volumes are available at discounted prices (enquire).
Technical Specifications:
Matrix: Separopore® 2B (agarose beads, 2%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as slurry in 20% ethanol
Chemical stability: Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride
Physical stability: Negligible volume variation due to changes in pH or ionic strength
Sterilization: Chemical
References:
Inhibition of fibrinogen binding to human platelets by S-nitroso-N-acetylcysteine. J Biol Chem. (1990) 265: 19028-34.
Nitric oxide released from activated platelets inhibits platelet recruitment. J Clin Invest. (1997) 100: 350-6.
Identification of the fibrinogen receptor on human platelets by photoaffinity labeling. J Biol Chem. (1982) 257: 8049-54.
An experimental model of pulmonary arterial granulomatous inflammation. Am J Pathol. (1973) 71: 365-72.
Identification and characterization of LAMP-1 as an activation-dependent platelet surface glycoprotein. J Biol Chem. (1990) 265: 18531-7.
Modulation of the arrestin-clathrin interaction in cells. Characterization of beta-arrestin dominant-negative mutants. J Biol Chem. (1997) 272: 32507-12.
Identification of lysosome-associated membrane protein-2 as an activation-dependent platelet surface glycoprotein. Blood. (1992) 80: 1470-5.
Inhibition of platelet adhesion to surfaces of extracorporeal circuits by disintegrins. RGD-containing peptides from viper venoms. Circulation. (1990) 82: 261-73.
Studies of the mechanism of tunicamycin in hibition of IgA and IgE secretion by plasma cells. J Biol Chem. (1977) 252: 4402-8.
Characterization of the binding of human tissue-type plasminogen activator to platelets. J Biol Chem. (1989) 264: 15869-74.
The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein. Proc Natl Acad Sci U S A. (1991) 88: 11212-6.
Lipoprotein(a) binds to human platelets and attenuates plasminogen binding and activation. Biochemistry. (1993) 32: 4628-33.
Regulation of glycoprotein IIB/IIIA exposure on platelets stimulated with alpha-thrombin. Blood. (1992) 79: 82-90.
Ultrastructural localization of human platelet thrombospondin, fibrinogen, fibronectin, and von Willebrand factor in frozen thin section. Blood. (1985) 65: 929-38.
Association of fibrin with the platelet cytoskeleton. J Biol Chem. (1984) 259: 5247-54.
Immunochemical analysis of normal and mutant forms of human pyruvate dehydrogenase. Biochem J. (1986) 239: 89-96.
Stimulation of mitogen-activated protein kinase and Na+/H+ exchanger in human platelets. Differential effect of phorbol ester and vasopressin. J Biol Chem. (1996) 271: 16494-9.
Tissue plasminogen activator promotes platelet disaggregation in plasma. J Clin Invest. (1987) 79: 1749-55.
Synthesis of cartilage-specific proteoglycan by suspension cultures of adult chondrocytes. Biochem J. (1977) 164: 269-72.
Identification of gallbladder mucin-bilirubin complex in human cholesterol gallstone matrix. Effects of reducing agents on in vitro dissolution of matrix and intact gallstones. J Clin Invest. (1985) 76: 439-45.
A role for prostaglandins and thromboxanes in the exposure of platelet fibrinogen receptors. J Clin Invest. (1981) 68: 981-7.
Protein kinase C and cyclic AMP regulate reversible exposure of binding sites for fibrinogen on the glycoprotein IIB-IIIA complex of human platelets. Biochem J. (1991) 273: 115-20.
Solid lipid nanocarriers as drug carriers II. Plasma stability and biodistribution of solid lipid nanoparticles containing the lipophilic prodrug 3'-azido-3'-deoxythymidine palmitate in mice. Int. J. Pharm. (1998) 174: 71-80.
The effect of molecular weight on heparin binding to platelets. Br J Haematol. (1990) 74: 306-12.
A study of the kinetics of ADP-triggered platelet shape change. Blood. (1984) 64: 896-906.
Plasma protein adsorption patterns on liposomes: establishment of analytical procedure. Electrophoresis. (1996) 17: 607-11.
Arrestin/clathrin interaction. Localization of the clathrin binding domain of nonvisual arrestins to the carboxy terminus. J Biol Chem. (1997) 272: 15011-6.
Characterization of recombinant and brain neuropsin, a plasticity-related serine protease. J Biol Chem. (1998) 273: 11189-96.
Immunologic and neuropharmacologic stimulation of mucous glycoprotein release from human airways in vitro. J Clin Invest. (1980) 66: 1400-8.
Multimeric structure of platelet factor VIII/von Willebrand factor: the presence of larger multimers and their reassociation with thrombin-stimulated platelets. Blood. (1982) 60: 1132-8.
Variations in the composition of bovine hip articular cartilage with distance from the articular surface. Biochem J. (1981) 195: 535-43.
Analysis of CD9, CD32 and p67 signalling: use of degranulated platelets indicates direct involvement of CD9 and p67 in integrin activation. Br J Haematol. (1997) 96: 275-86.
Platelet stimulation releases a cAMP-dependent protein kinase that specifically phosphorylates a plasma protein. Proc Natl Acad Sci U S A. (1988) 85: 7541-5.
Heparin binding to resting and activated platelets. Blood. (1989) 74: 238-43.
Hydrogen peroxide is involved in collagen-induced platelet activation. Blood. (1998) 91: 484-90.
Stimulation of platelet activation and aggregation by a carboxyl-terminal peptide from thrombospondin binding to the integrin-associated protein receptor. J Biol Chem. (1997) 272: 1323-30.
Activation of soluble guanylate cyclase from rat lung by incubation or by hydrogen peroxide. J Biol Chem. (1976) 251: 7304-12.
Purification and properties of a kinase which phosphorylates and inactivates acetyl-CoA carboxylase. J Biol Chem. (1982) 257: 1897-901.
Association of coagulation factor V with the platelet cytoskeleton. J Biol Chem. (1982) 257: 4557-63.
Protein kinase C mediates translocation of type II phosphatidylinositol 5-phosphate 4-kinase required for platelet alpha-granule secretion. J Biol Chem. (2003) 278: 8126-34.
Cyclopiazonic acid and thapsigargin induce platelet aggregation resulting from Ca2+ influx through Ca2+ store-activated Ca2+-channels. Eur J Pharmacol. (1998) 341: 343-7.
Cation gradient dependence of the steps in thrombin stimulation of human platelets. J Biol Chem. (1984) 259: 1502-8.
Regulation of rat liver acetyl-CoA carboxylase: Regulation of phosphorylation and inactivation of acetyl-CoA carboxylase by the adenylate energy charge. J Biol Chem. (1980) 255: 2308-14.
Ultrastructural localization of coagulation factor V in human platelets. Blood. (1986) 68: 244-9.
Detection of soluble class I molecules (non HLA-A or HLA-B) in serum, spleen membranes and lymphocytes in culture. Eur J Immunol. (1989) 19: 1835-9.
Immunolocalization of beta 1 integrins in platelets and platelet-derived microvesicles. Blood. (1993) 82: 1197-203.
Coenzyme A activation of acetyl-CoA carboxylase. J Biol Chem. (1981) 256: 2289-96.
The actin cytoskeleton differentially regulates platelet alpha-granule and dense-granule secretion. Blood. (2005) 105: 3879-87.
The platelet cytoskeleton contains elements of the prothrombinase complex. J Biol Chem. (1984) 259: 6947-51.
Comparison of carbohydrate-containing substances from non-calcified and calcified portions of bovine costal cartilage. J Biochem. (1979) 85: 1067-73.
Continuous binding of the PAF molecule to its receptor is necessary for the long-term aggregation of platelets. Am J Physiol. (1998) 274: C47-57.
Biochemical isolation of a membrane microdomain from resting platelets highly enriched in the plasma membrane glycoprotein CD36. Biochem J. (1996) 319: 67-72.
Labeling of human platelet plasma membrane thromboxane A2/prostaglandin H2 receptors using SQB, a novel biotinylated receptor probe. Biochem Pharmacol. (1996) 52: 763-70.
Localization of dipeptidyl aminopeptidase yscIV in the plasma membrane of Saccharomyces cerevisiae. J Gen Microbiol. (1990) 136: 419-23.
Staphylococcus aureus alpha-toxin attack on human platelets promotes assembly of the prothrombinase complex. J Biol Chem. (1990) 265: 14377-81.
Evidence for inhibitors of the cell surface protease guanidinobenzoatase. J Enzyme Inhib. (1986) 1: 127-37.
Interaction of T lymphocytes and macrophages with cultured vascular endothelial cells: attachment, invasion, and subsequent degradation of the subendothelial extracellular matrix. J Cell Physiol. (1984) 118: 169-78.
Assembly of proteoglycan aggregates in cultures of chondrocytes from bovine tracheal cartilage. Biochem J. (1981) 199: 17-29.
Rat liver microsomal and lysosomal beta-glucuronidases differ in both carbohydrate and amino acid compositions. Proc Natl Acad Sci U S A. (1978) 75: 3080-4.
Pattern of endogenous lectins in a human epithelial tumor. Cancer Res. (1985) 45: 253-7.
Related products:
Separopore® 2B-CL (SKU # 20181053)
Separopore® 2B-MB (Macrobeads) (SKU # 20181054)
Separopore® 4B (SKU # 20181031)
Separopore® 4B-CL (SKU # 20181032)
Separopore® 4B-MB (Macrobeads) (SKU #20181033)
Separopore® 6B (SKU # 20181043)
Separopore® 6B-CL (SKU # 20181036)
Separopore® 6B-MB (Macrobeads) (SKU # 20181035)
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®
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