Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181032
Application:
Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides good separation over a wide molecular weight range. Larger volumes available at discounted prices (enquire).
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
pH stability: 3 – 12
Flow Specifications: 70 – 140 cm / h
Supplied as a slurry in 20% ethanol
Chemical stability: Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride
Physical stability: Negligible volume variation due to changes in pH or ionic strength
Sterilization: Chemical
References:
Affinity-column-mediated immunoenzymometric assays: influence of affinity-column ligand and valency of antibody-enzyme conjugates. Clin Chem. (1984) 30: 1494-8.
Multiple gene-like sequences related to the rabbit hepatic progesterone 21-hydroxylase cytochrome P-450 1. J Biol Chem. (1985) 260: 13347-54.
Proteoglycans from adult human gingival epithelium. Biochem J. (1979) 183: 467-70.
Characterization of a major fibroblast cell surface glycoprotein. Biochemistry. (1977) 16: 5552-9.
Photocoagulation of human plasma: acyl serine proteinase photochemistry. Photochem Photobiol. (1990) 51: 37-43.
The immobilization of mitochondrial malate dehydrogenase on Sepharose beads and the demonstration of catalytically active subunits. J Biol Chem. (1981) 256: 2383-8.
Salt-independent binding of antibodies from human serum to thiophilic heterocyclic ligands. J Chromatogr B Biomed Sci Appl. (1998) 709: 189-96.
Identification of a polymorphically expressed member of the human cytochrome P-450III family. Mol Pharmacol. (1989) 36: 97-105.
Two monoclonal antibodies recognizing different epitopes on rat cytochrome IIB1 react with human IIE1. Mol Pharmacol. (1992) 41: 76-82.
Isolation of a five-polypeptide cytochrome b-f complex from spinach chloroplasts. J Biol Chem. (1983) 258: 10348-54.
The 110,000-dalton actin- and calmodulin-binding protein from intestinal brush border is a myosin-like ATPase. J Biol Chem. (1984) 259: 14128-35.
Biotin-binding protein from egg yolk. A protein distinct from egg white avidin. J Biol Chem. (1978) 253: 6979-82.
Degradation of heparin proteoglycan in cultured mouse mastocytoma cells. Biochem J. (1987) 246: 409-15.
Fibronectin in human gallbladder bile: cholesterol pronucleating and/or mucin "link" protein? Am J Physiol. (1994) 267: G393-400.
Monoclonal and polyclonal antibodies recognizing acetaldehyde-protein adducts. Biochem Pharmacol. (1998) 56: 1515-23.
Related products:
Separopore® 2B-CL (SKU # 20181053)
Separopore® 2B (SKU # 20181045)
Separopore® 2B-MB (Macrobeads) (SKU # 20181054)
Separopore® 4B (SKU # 20181031)
Separopore® 4B-MB (Macrobeads) (SKU #20181033)
Separopore® 6B (SKU # 20181043)
Separopore® 6B-CL (SKU # 20181036)
Separopore® 6B-MB (Macrobeads) (SKU # 20181035)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides good separation over a wide molecular weight range. Larger volumes available at discounted prices (enquire).
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
pH stability: 3 – 12
Flow Specifications: 70 – 140 cm / h
Supplied as a slurry in 20% ethanol
Chemical stability: Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride
Physical stability: Negligible volume variation due to changes in pH or ionic strength
Sterilization: Chemical
References:
Affinity-column-mediated immunoenzymometric assays: influence of affinity-column ligand and valency of antibody-enzyme conjugates. Clin Chem. (1984) 30: 1494-8.
Multiple gene-like sequences related to the rabbit hepatic progesterone 21-hydroxylase cytochrome P-450 1. J Biol Chem. (1985) 260: 13347-54.
Proteoglycans from adult human gingival epithelium. Biochem J. (1979) 183: 467-70.
Characterization of a major fibroblast cell surface glycoprotein. Biochemistry. (1977) 16: 5552-9.
Photocoagulation of human plasma: acyl serine proteinase photochemistry. Photochem Photobiol. (1990) 51: 37-43.
The immobilization of mitochondrial malate dehydrogenase on Sepharose beads and the demonstration of catalytically active subunits. J Biol Chem. (1981) 256: 2383-8.
Salt-independent binding of antibodies from human serum to thiophilic heterocyclic ligands. J Chromatogr B Biomed Sci Appl. (1998) 709: 189-96.
Identification of a polymorphically expressed member of the human cytochrome P-450III family. Mol Pharmacol. (1989) 36: 97-105.
Two monoclonal antibodies recognizing different epitopes on rat cytochrome IIB1 react with human IIE1. Mol Pharmacol. (1992) 41: 76-82.
Isolation of a five-polypeptide cytochrome b-f complex from spinach chloroplasts. J Biol Chem. (1983) 258: 10348-54.
The 110,000-dalton actin- and calmodulin-binding protein from intestinal brush border is a myosin-like ATPase. J Biol Chem. (1984) 259: 14128-35.
Biotin-binding protein from egg yolk. A protein distinct from egg white avidin. J Biol Chem. (1978) 253: 6979-82.
Degradation of heparin proteoglycan in cultured mouse mastocytoma cells. Biochem J. (1987) 246: 409-15.
Fibronectin in human gallbladder bile: cholesterol pronucleating and/or mucin "link" protein? Am J Physiol. (1994) 267: G393-400.
Monoclonal and polyclonal antibodies recognizing acetaldehyde-protein adducts. Biochem Pharmacol. (1998) 56: 1515-23.
Related products:
Separopore® 2B-CL (SKU # 20181053)
Separopore® 2B (SKU # 20181045)
Separopore® 2B-MB (Macrobeads) (SKU # 20181054)
Separopore® 4B (SKU # 20181031)
Separopore® 4B-MB (Macrobeads) (SKU #20181033)
Separopore® 6B (SKU # 20181043)
Separopore® 6B-CL (SKU # 20181036)
Separopore® 6B-MB (Macrobeads) (SKU # 20181035)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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