Shigella dysenteriae serotype 1 (clone EBS-I-106), anti-S. dysenteriae

Source:
A BALB/c mouse was immunized with total sonicate of Shigella dysenteriae NCTC 4837.
Fusion partner: NS-1.

Specifications:
EBS-I-106 reacts with a soluble extracted antigen from Shigella dysenteriae S1 in EIA. Shigellae are Gram-negative, non-spore-forming, facultative anaerobic, non-motile bacteria. S.dysenteriaeis a species of the rod-shaped bacterial genus Shigella. This microbe is a normal inhabitant of the human gastrointestinal tract and can cause shigellosis (bacillary dysentery). This is the most severe dysentery mainly because of its potent and deadly Shiga toxin. Shiga toxins work by inhibiting protein synthesis in the host cells. After entering a cell, the Shiga toxin acts as an N-glycosidase, cleaving several nucleobases from the RNA that comprises the ribosome, thereby halting protein synthesis. The toxin has two subunits: A, which is internalized and cleaved into two parts, one of which binds to the ribosome, disrupting protein synthesis; and B, a pentamer that binds to specific glycolipids on the host cell, specifically globotriaosylceramide. S.dysenteriaeis spread through contaminated water and foodstrains are resistant to several antibiotics because of the presence of R factors in plasmids.

Species reactivity:
Positive: S. dysenteriae.

Applications:
Detection of S. dysenteriae. ELISA: +
Frozen sections: +
Immunofluorescence: +

Positive control: S. dysenteriae S1 extract or infected cells or tissue, NCTC 4837.

References:
- Feng, L, et al, Microb. Pathog.36: 109-115 (2003).
- Dutta, S, et al, Microbiol. Immunol. 48: 965-969(2004)