Simian α2M (Alpha-2-Macroglobulin) ELISA Kit
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In stock
SKU
ELK8292
Catalog Number: ELK8292
Reactivity: Simian
Standard Range: 7.82-500 ng/mL
Sensitivity: 3.5 ng/mL
Information
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Reactivity: Simian
Standard Range: 7.82-500 ng/mL
Sensitivity: 3.5 ng/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin(α2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin(α2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin(α2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin(α2M) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Tumor immunity, Infection immunity, Hematology
Alternative Names:
α2MG, α2Mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type: Sandwich
Standard: 500 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin(α2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin(α2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin(α2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin(α2M) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Tumor immunity, Infection immunity, Hematology
Alternative Names:
α2MG, α2Mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type: Sandwich
Standard: 500 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
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