Simian NGF (Nerve Growth Factor) ELISA Kit
€0.00
In stock
SKU
ELK7900
Catalog Number: ELK7900
Reactivity: Simian
Standard Range: 15.63-1000 pg/mL
Sensitivity: 6.2 pg/mL
Information
Request Manual
Questions? Contact us!
Reactivity: Simian
Standard Range: 15.63-1000 pg/mL
Sensitivity: 6.2 pg/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor(NGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nerve Growth Factor(NGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nerve Growth Factor(NGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nerve Growth Factor(NGF) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Cytokine, Neuro science
Alternative Names:
NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
Sample Type:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Type: Sandwich
Standard: 1000 pg/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor(NGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nerve Growth Factor(NGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nerve Growth Factor(NGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nerve Growth Factor(NGF) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Cytokine, Neuro science
Alternative Names:
NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
Sample Type:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Type: Sandwich
Standard: 1000 pg/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
Is Featured? | No |
---|
Write Your Own Review