SOD Activity Assay Kit

Superoxide dismutases (SOD) catalyse the breakdown of superoxide radicals and provide the first line of defense against oxygen toxicity.

SOD Assay Kit, superoxide ions are generated from the conversion of xanthine and O2 to uric acid and H2O2 by Xanthine Oxidase (XO). The superoxide anion then coverts the tetrazolium salt WST-1 to the colored product WST-1 formazan.

Absorbance is then measured at 450 nm using a standard microplate reader. Addition of SOD to this reaction reduces superoxide ion levels, thereby lowering the rate of WST-1 formazan formation. SOD activity in the experimental sample is measured as the percent inhibition of the rate of WST-1 formazan formation.

Advantages & Features
- Easy-to-use.
- Fast protocol.

Includes
- 1 mL WST-1 Reagent
- 20 mL SOD assay buffer
- 10 mL SOD dilution buffer
- 30 µL SOD enzyme solution
- 1 mg SOD standard (3000 U/mg)

Applications
Quantitative determination of Superoxide Dismutase (SOD) enzyme activity.

Shipping & Storage
Shipping: Gel pack.
Storage: The SOD Standard should be stored at ‐20 °C. All other components of this kit are stable at 4 °C until the kit expiration date.
Recommendations: WST-1 reagent is light sensitive and should be maintained in amber tubes.