Staphylococcus aureus (Nuc) DNA and Staphylococcus aureus (PVL) PCR Kit CE/IVD
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In stock
SKU
RealCycler SAPV-
Catalog Number: RealCycler SAPV-
1 Intended use:
RealCycler SAPV is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from Staphylococcus aureus and Panton-Valentine (PVL) leucocidine toxin simultaneously in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
The staphylococcus aureus pathogen can be quantified when this product is used in combination with the corresponding calibrator (reference RealCycler AURQ.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Panton-Valentine leucocidinetoxin(PVL) is detected in the corresponding channel of FAM fluorophore, CHIC is detected in the corresponding channel of Alx532 or HEX fluorophore and Staphylococcus aureus is detected in the corresponding channel of Alx647 or Cy5 fluorophore.
3 Technical specifications
Sensitivity
- Panton-Valentine leucocidine toxin (PVL): 100 copies/μL and
- Staphylococcus aureus: 100 copies/μL.
Specificity
- Panton-Valentine leucocidine toxin: PVL gene and
- Staphylococcus aureus: NUC gene.
4 Contents
RealCycler SAPV includes the AmpliMix and a SAPV DNA Positive Control, which contains S. aureus PVL+ DNA.
All reagents are ready to use without adding or rebuilding any component.
May 2014: Poster at the 24th European Congress of Clinical Microbiology and Infectious Diseases (ESCMID), Barcelona "Improved Laboratory Reporting Times And Clinical Impact For Panton-Valentine Leukocidin Testing Using The Progenie Realcycler SAPV Real-time P.C.R. Assay" Link
RealCycler SAPV is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from Staphylococcus aureus and Panton-Valentine (PVL) leucocidine toxin simultaneously in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
The staphylococcus aureus pathogen can be quantified when this product is used in combination with the corresponding calibrator (reference RealCycler AURQ.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Panton-Valentine leucocidinetoxin(PVL) is detected in the corresponding channel of FAM fluorophore, CHIC is detected in the corresponding channel of Alx532 or HEX fluorophore and Staphylococcus aureus is detected in the corresponding channel of Alx647 or Cy5 fluorophore.
3 Technical specifications
Sensitivity
- Panton-Valentine leucocidine toxin (PVL): 100 copies/μL and
- Staphylococcus aureus: 100 copies/μL.
Specificity
- Panton-Valentine leucocidine toxin: PVL gene and
- Staphylococcus aureus: NUC gene.
4 Contents
RealCycler SAPV includes the AmpliMix and a SAPV DNA Positive Control, which contains S. aureus PVL+ DNA.
All reagents are ready to use without adding or rebuilding any component.
May 2014: Poster at the 24th European Congress of Clinical Microbiology and Infectious Diseases (ESCMID), Barcelona "Improved Laboratory Reporting Times And Clinical Impact For Panton-Valentine Leukocidin Testing Using The Progenie Realcycler SAPV Real-time P.C.R. Assay" Link
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