Strep II (clone ), anti-all
€305.00
In stock
SKU
MB2017
Background:
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
Alternative Name:
Strep-Tag II, Strep II
Application Dilution: WB: 1:5000~1:10000, IF: 1:200
Specificity: Strep Ⅱtag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal Strep Ⅱ- tagged proteins.
Immunogen:
Synthetic peptide Strep II Tag.
Purification & Purity:
Affinity-chromatography
Format:
Mouse IgG1 in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
Alternative Name:
Strep-Tag II, Strep II
Application Dilution: WB: 1:5000~1:10000, IF: 1:200
Specificity: Strep Ⅱtag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal Strep Ⅱ- tagged proteins.
Immunogen:
Synthetic peptide Strep II Tag.
Purification & Purity:
Affinity-chromatography
Format:
Mouse IgG1 in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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