Streptavidin Green Separopore® 4B Microbeads viewIP™
€0.00
In stock
SKU
BW-20181109
Application:
The green-colored agarose bead affinity matrix contains immobilized Streptavidin. It is convenient for affinity capture (pull-down) of biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands. Comparable performance to non-colored affinity gels, viewIP™ Streptavidin Green-Separopore® 4B-CL greatly reduce the risk of pellet loss comparable performance to non-colored affinity gels and allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample loss.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Streptavidin
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size (range): 52 – 165 µm
Exclusion limit: approximately 2 x 107
Matrix activation: Cyanogen Bromide
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Supplied as 50% suspension in PBS, containing 50% glycerol
Storage: 2 – 8 °C, long term can be stored -20 °C
References:
Triplex DNA-binding proteins are associated with clinical outcomes revealed by proteomic measurements in patients with colorectal cancer. Mol Cancer. (2012) 11: 38.
In vitro characterization of organophosphorus compound hydrolysis by native and recombinant human prolidase. Toxicol In Vitro. (2013) 27: 499-506.
Oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins. J Biotechnol. (2012) 158: 97-103.
High-performance liquid chromatography-electrospray ionization-mass spectrometry ligand fishing assay: a method for screening triplex DNA binders from natural plant extracts. Anal Chem. (2012) 84: 2562-8.
Rapid S-nitrosylation of actin by NO-generating donors and in inflammatory pain model mice. Mol Pain. (2011) 7: 101.
Quercetin suppresses cyclooxygenase-2 expression and angiogenesis through inactivation of P300 signaling. PLoS One. (2011) 6: e22934.
Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3. Mol Cell Proteomics. (2011) 10: M110.006007.
The CCAAT/enhancer binding protein (C/EBP) δ is differently regulated by fibrillar and oligomeric forms of the Alzheimer amyloid-β peptide. J Neuroinflammation. (2011) 8: 34.
Acyl-biotinyl exchange chemistry and mass spectrometry-based analysis of palmitoylation sites of in vitro palmitoylated rat brain tubulin. Protein J. (2010) 29: 531-7.
A possible key molecule for the invasion of the Plasmodium berghei ookinetes into the midgut epithelium of Anopheles gambiae mosquitoes. In Vivo. (2010) 24: 271-80.
Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis. J Neurosci Res. (2009) 87: 2881-9.
Proteomic analysis of the lymphocyte plasma membrane using cell surface biotinylation and solution-phase isoelectric focusing. Methods Mol Biol. (2009) 528: 135-40.
Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research. PLoS One. (2008) 3: e3822.
Mechanism of 3,4-methylenedioxymethamphetamine (MDMA, ecstasy)-mediated mitochondrial dysfunction in rat liver. Proteomics. (2008) 8: 3906-18.
Oxidative inactivation of key mitochondrial proteins leads to dysfunction and injury in hepatic ischemia reperfusion. Gastroenterology. (2008) 135: 1344-57.
A requirement for thioredoxin in redox-sensitive modulation of T-cadherin expression in endothelial cells. Biochem J. (2008) 416: 271-80.
An enzyme capture assay for analysis of active hyaluronan synthases. Anal Biochem. (2007) 371: 62-70.
Identification of novel surface proteins of Anaplasma phagocytophilum by affinity purification and proteomics. J Bacteriol. (2007) 189: 7819-28.
Unbiased identification of cysteine S-nitrosylation sites on proteins. Nat Protoc. (2007) 2: 1685-91.
Palmitoylated proteins: purification and identification. Nat Protoc. (2007) 2: 1573-84.
Surface-exposed proteins of Ehrlichia chaffeensis. Infect Immun. (2007) 75: 3833-41.
Reversible site-specific tagging of enzymatically synthesized RNAs using aldehyde-hydrazine chemistry and protease-cleavable linkers. Nucleic Acids Res. (2007) 35: e25.
Recombinant soluble forms of extracellular TLR4 domain and MD-2 inhibit lipopolysaccharide binding on cell surface and dampen lipopolysaccharide-induced pulmonary inflammation in mice. J Immunol. (2006) 177: 8133-9.
Inactivation of oxidized and S-nitrosylated mitochondrial proteins in alcoholic fatty liver of rats. Hepatology. (2006) 44: 1218-30.
Rapid and selective isolation of beta-xylosidase through an activity-based chemical approach. Biotechnol J. (2006) 1: 197-202.
Analysis of protein-DNA binding by streptavidin-agarose pulldown. Methods Mol Biol. (2006) 338: 281-90.
A rapid enzyme-linked assay for ADAMTS-13. J Thromb Haemost. (2006) 4: 129-36.
Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells. Proteomics. (2006) 6: 1250-60.
The biotin-capture lipid affinity assay: a rapid method for determining lipid binding parameters for apolipoproteins. J Lipid Res. (2006) 47: 440-9.
Specific N-terminal biotinylation of a protein in vitro by a chemically modified tRNA(fmet) can support the native activity of the translated protein. J Biosci Bioeng. (2001) 92: 149-53.
The utility of N,N-biotinyl glutathione disulfide in the study of protein S-glutathiolation. Mol Cell Proteomics. (2006) 5: 215-25.
Effect of nuclear actin on endothelial nitric oxide synthase expression. Arterioscler Thromb Vasc Biol. (2005) 25: 2509-14.
Widespread sulfenic acid formation in tissues in response to hydrogen peroxide. Proc Natl Acad Sci U S A. (2004) 101: 17982-7.
Identification of oxidized mitochondrial proteins in alcohol-exposed human hepatoma cells and mouse liver. Proteomics. (2004) 4: 3401-12.
Development of an enzymatic method for site-specific incorporation of desthiobiotin to recombinant proteins in vitro. Anal Biochem. (2004) 331: 340-8.
Insulin-like growth factor binding protein-3 interacts with autocrine motility factor/phosphoglucose isomerase (AMF/PGI) and inhibits the AMF/PGI function. Cancer Res. (2004) 64: 2516-22.
Quantitative analysis of binding of transcription factor complex to biotinylated DNA probe by a streptavidin-agarose pulldown assay. Anal Biochem. (2003) 323: 12-8.
Reversible cysteine-targeted oxidation of proteins during renal oxidative stress. J Am Soc Nephrol. (2003) 14: S290-6.
The human telomere-associated protein TIN2 stimulates interactions between telomeric DNA tracts in vitro. EMBO Rep. (2003) 4: 685-91.
EliCell assay for the detection of released cytokines from eosinophils. J Immunol Methods. (2003) 276: 227-37.
Annexin II is present on renal epithelial cells and binds calcium oxalate monohydrate crystals. J Am Soc Nephrol. (2003) 14: 289-97.
Up-regulation of p300 binding and p50 acetylation in tumor necrosis factor-alpha-induced cyclooxygenase-2 promoter activation. J Biol Chem. (2003) 278: 4770-7.
Nuclear convergence of the TGFbeta and cAMP signal transduction pathways in murine embryonic palate mesenchymal cells. Cell Signal. (2003) 15: 235-42.
Glyceraldehyde phosphate dehydrogenase oxidation during cardiac ischemia and reperfusion. J Mol Cell Cardiol. (2002) 34: 1549-60.
Partial reconstitution of human RNase P in HeLa cells between its RNA subunit with an affinity tag and the intact protein components. Nucleic Acids Res. (2002) 30: 3706-11.
In vitro selection of mRNA display libraries containing an unnatural amino acid. J Am Chem Soc. (2002) 124: 9972-3.
A nonpermeant biotin derivative gains access to the parasitophorous vacuole in Plasmodium falciparum-infected erythrocytes permeabilized with streptolysin O. J Biol Chem. (2002) 277: 40005-11.
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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®, ViewIP™
The green-colored agarose bead affinity matrix contains immobilized Streptavidin. It is convenient for affinity capture (pull-down) of biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands. Comparable performance to non-colored affinity gels, viewIP™ Streptavidin Green-Separopore® 4B-CL greatly reduce the risk of pellet loss comparable performance to non-colored affinity gels and allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample loss.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Streptavidin
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size (range): 52 – 165 µm
Exclusion limit: approximately 2 x 107
Matrix activation: Cyanogen Bromide
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Supplied as 50% suspension in PBS, containing 50% glycerol
Storage: 2 – 8 °C, long term can be stored -20 °C
References:
Triplex DNA-binding proteins are associated with clinical outcomes revealed by proteomic measurements in patients with colorectal cancer. Mol Cancer. (2012) 11: 38.
In vitro characterization of organophosphorus compound hydrolysis by native and recombinant human prolidase. Toxicol In Vitro. (2013) 27: 499-506.
Oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins. J Biotechnol. (2012) 158: 97-103.
High-performance liquid chromatography-electrospray ionization-mass spectrometry ligand fishing assay: a method for screening triplex DNA binders from natural plant extracts. Anal Chem. (2012) 84: 2562-8.
Rapid S-nitrosylation of actin by NO-generating donors and in inflammatory pain model mice. Mol Pain. (2011) 7: 101.
Quercetin suppresses cyclooxygenase-2 expression and angiogenesis through inactivation of P300 signaling. PLoS One. (2011) 6: e22934.
Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3. Mol Cell Proteomics. (2011) 10: M110.006007.
The CCAAT/enhancer binding protein (C/EBP) δ is differently regulated by fibrillar and oligomeric forms of the Alzheimer amyloid-β peptide. J Neuroinflammation. (2011) 8: 34.
Acyl-biotinyl exchange chemistry and mass spectrometry-based analysis of palmitoylation sites of in vitro palmitoylated rat brain tubulin. Protein J. (2010) 29: 531-7.
A possible key molecule for the invasion of the Plasmodium berghei ookinetes into the midgut epithelium of Anopheles gambiae mosquitoes. In Vivo. (2010) 24: 271-80.
Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis. J Neurosci Res. (2009) 87: 2881-9.
Proteomic analysis of the lymphocyte plasma membrane using cell surface biotinylation and solution-phase isoelectric focusing. Methods Mol Biol. (2009) 528: 135-40.
Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research. PLoS One. (2008) 3: e3822.
Mechanism of 3,4-methylenedioxymethamphetamine (MDMA, ecstasy)-mediated mitochondrial dysfunction in rat liver. Proteomics. (2008) 8: 3906-18.
Oxidative inactivation of key mitochondrial proteins leads to dysfunction and injury in hepatic ischemia reperfusion. Gastroenterology. (2008) 135: 1344-57.
A requirement for thioredoxin in redox-sensitive modulation of T-cadherin expression in endothelial cells. Biochem J. (2008) 416: 271-80.
An enzyme capture assay for analysis of active hyaluronan synthases. Anal Biochem. (2007) 371: 62-70.
Identification of novel surface proteins of Anaplasma phagocytophilum by affinity purification and proteomics. J Bacteriol. (2007) 189: 7819-28.
Unbiased identification of cysteine S-nitrosylation sites on proteins. Nat Protoc. (2007) 2: 1685-91.
Palmitoylated proteins: purification and identification. Nat Protoc. (2007) 2: 1573-84.
Surface-exposed proteins of Ehrlichia chaffeensis. Infect Immun. (2007) 75: 3833-41.
Reversible site-specific tagging of enzymatically synthesized RNAs using aldehyde-hydrazine chemistry and protease-cleavable linkers. Nucleic Acids Res. (2007) 35: e25.
Recombinant soluble forms of extracellular TLR4 domain and MD-2 inhibit lipopolysaccharide binding on cell surface and dampen lipopolysaccharide-induced pulmonary inflammation in mice. J Immunol. (2006) 177: 8133-9.
Inactivation of oxidized and S-nitrosylated mitochondrial proteins in alcoholic fatty liver of rats. Hepatology. (2006) 44: 1218-30.
Rapid and selective isolation of beta-xylosidase through an activity-based chemical approach. Biotechnol J. (2006) 1: 197-202.
Analysis of protein-DNA binding by streptavidin-agarose pulldown. Methods Mol Biol. (2006) 338: 281-90.
A rapid enzyme-linked assay for ADAMTS-13. J Thromb Haemost. (2006) 4: 129-36.
Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells. Proteomics. (2006) 6: 1250-60.
The biotin-capture lipid affinity assay: a rapid method for determining lipid binding parameters for apolipoproteins. J Lipid Res. (2006) 47: 440-9.
Specific N-terminal biotinylation of a protein in vitro by a chemically modified tRNA(fmet) can support the native activity of the translated protein. J Biosci Bioeng. (2001) 92: 149-53.
The utility of N,N-biotinyl glutathione disulfide in the study of protein S-glutathiolation. Mol Cell Proteomics. (2006) 5: 215-25.
Effect of nuclear actin on endothelial nitric oxide synthase expression. Arterioscler Thromb Vasc Biol. (2005) 25: 2509-14.
Widespread sulfenic acid formation in tissues in response to hydrogen peroxide. Proc Natl Acad Sci U S A. (2004) 101: 17982-7.
Identification of oxidized mitochondrial proteins in alcohol-exposed human hepatoma cells and mouse liver. Proteomics. (2004) 4: 3401-12.
Development of an enzymatic method for site-specific incorporation of desthiobiotin to recombinant proteins in vitro. Anal Biochem. (2004) 331: 340-8.
Insulin-like growth factor binding protein-3 interacts with autocrine motility factor/phosphoglucose isomerase (AMF/PGI) and inhibits the AMF/PGI function. Cancer Res. (2004) 64: 2516-22.
Quantitative analysis of binding of transcription factor complex to biotinylated DNA probe by a streptavidin-agarose pulldown assay. Anal Biochem. (2003) 323: 12-8.
Reversible cysteine-targeted oxidation of proteins during renal oxidative stress. J Am Soc Nephrol. (2003) 14: S290-6.
The human telomere-associated protein TIN2 stimulates interactions between telomeric DNA tracts in vitro. EMBO Rep. (2003) 4: 685-91.
EliCell assay for the detection of released cytokines from eosinophils. J Immunol Methods. (2003) 276: 227-37.
Annexin II is present on renal epithelial cells and binds calcium oxalate monohydrate crystals. J Am Soc Nephrol. (2003) 14: 289-97.
Up-regulation of p300 binding and p50 acetylation in tumor necrosis factor-alpha-induced cyclooxygenase-2 promoter activation. J Biol Chem. (2003) 278: 4770-7.
Nuclear convergence of the TGFbeta and cAMP signal transduction pathways in murine embryonic palate mesenchymal cells. Cell Signal. (2003) 15: 235-42.
Glyceraldehyde phosphate dehydrogenase oxidation during cardiac ischemia and reperfusion. J Mol Cell Cardiol. (2002) 34: 1549-60.
Partial reconstitution of human RNase P in HeLa cells between its RNA subunit with an affinity tag and the intact protein components. Nucleic Acids Res. (2002) 30: 3706-11.
In vitro selection of mRNA display libraries containing an unnatural amino acid. J Am Chem Soc. (2002) 124: 9972-3.
A nonpermeant biotin derivative gains access to the parasitophorous vacuole in Plasmodium falciparum-infected erythrocytes permeabilized with streptolysin O. J Biol Chem. (2002) 277: 40005-11.
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View-IP™ Protein A Red-Separopore® 4B-CL (SKU # 20181041)
View-IP™ Protein A Green-Separopore® 4B-CL (SKU # 20181108)
View-IP™ Protein A Blue-Separopore® 4B-CL (SKU # 20181105)
View-IP™ Protein G Red-Separopore® 4B-CL (SKU # 20181118)
View-IP™ Streptavidin Red-Separopore® 4B-CL (SKU # 20181103)
View-IP™ Streptavidin Blue-Separopore® 4B-CL (SKU # 20181106)
View-IP™ His-tag Nickel Red-Separopore® 6B-CL (SKU # 20181117)
View-IP™ Glutathione Red-Separopore® 4B-CL (SKU # 20181116)
View-IP™ Calmodulin Red-Separopore® 4B-CL (SKU # 20181104)
View-IP™ Calmodulin Green-Separopore® 4B-CL (SKU # 20181110)
View-IP™ Calmodulin Blue-Separopore® 4B-CL (SKU # 20181107)
View-IP™ Anti-HA Red-Separopore® 4B-CL (SKU # 20181115)
View-IP™ Anti-DYKDDDDK (FLAG) Red-Separopore® 4B-CL (SKU # 20181114)
View-IP™ Anti-cMyc Red-Separopore® 4B-CL (SKU # 20181113)
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®, ViewIP™
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