Streptavidin Separopore® 4B
€0.00
In stock
SKU
BW-20181037
Application:
Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability.
The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography Application: .
The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligandand the sample.
Strptavidin-Separopore® 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
It can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Separopore from which the antigen can be eluted.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Native Streptavidin (from Streptomyces avidinii)
Matrix: Separopore 4B (agarose beads, 4%)
Particle size range: 53 -180 µm
Matrix activation: Cyanogen bromide
Ligand density: 2 mg streptavidin / ml drained gel
Binding capacity: >20 µg biotin / ml drained gel
Exclusion limit: ~ 2 x 107
pH stability: 3 – 10
Binding buffer: 50 mM ammonium carbonate, 0.5 M NaCl, pH 10
Elution buffer: 50 mM ammonium acetate, 0.5 M NaCl, pH 4
Supplied as suspension in buffered saline solution with 0.05% sodium azide.
Storage: 2 – 8 °C
References:
Ku protein as the main cellular target of cell-surface-bound circulating DNA. Expert Opin Biol Ther. (2012) 12: S35-41.
Purification of the proprotein convertase furin by affinity chromatography based on PC-specific inhibitors. Biol Chem. (2011) 392: 973-81.
Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: The example of the sarcoplasmic reticulum Ca(2+)-ATPase. Methods Mol Biol. (2010) 601: 247-67.
Binding characteristics of sperm with recombinant human zona pellucida glycoprotein-3 coated beads. Indian J Med Res. (2009) 130: 37-43.
The glutamic acid decarboxylase 65 immunoglobulin G subclass profile differs between adult-onset type 1 diabetes and latent autoimmune diabetes in adults (LADA) up to 3 years after clinical onset. Clin Exp Immunol. (2009) 157: 255-60.
Quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins. Methods Mol Biol. (2008) 418: 89-100.
Quantification of diphtheria toxin mediated ADP-ribosylation in a solid-phase assay. Clin Chem. (2007) 53: 1676-83.
Determination of glutamic acid decarboxylase antibodies (GADA) IgG subclasses - comparison of three immunoprecipitation assays (IPAs). Clin Exp Immunol. (2007) 150: 68-74.
Factor H is a dermatan sulfate-binding protein: identification of a dermatan sulfate-mediated protease that cleaves factor H. J Biochem. (2005) 137: 225-33.
In situ imaging and isolation of proteins using dsDNA oligonucleotides. Nucleic Acids Res. (2004) 32: e165.
Biotinylated tags for recovery and characterization of ribonucleoprotein complexes. Biotechniques. (2004) 37:608-10.
Purification of biotinylated proteins on streptavidin resin: a protocol for quantitative elution. Proteomics. (2004) 4: 2296-9.
The effect of free DNA on the interactions of the estrogen receptor bound to hormone, partial antagonist or pure antagonist with target DNA. Eur J Biochem. (1995) 231: 204-13.
Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes. Biotechnology (N Y). (1995) 13: 155-60.
Identification of a receptor candidate for the carboxyl-terminal cell binding domain of thrombospondins. J Biol Chem. (1994) 269: 29650-7.
Biotinylation of transducin and G0 from bovine brain. J Protein Chem. (1991) 10: 325-32.
Biotin-dependent expression of the asialoglycoprotein receptor in HepG2. J Biol Chem. (1988) 263: 11280-3.
Effect of plasma biotinyl-peptides on biotinidase activity. Biofactors. (1988) 1: 179-85.
Specific isolation of surface glycoproteins from intact cells by biotinylated concanavalin A and immobilized streptavidin. Anal Biochem. (1986) 156: 463-72.
Related products:
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Calmodulin-Separopore® 4B (SKU # 20181001)
E. coli Protein-Separopore® 4B (SKU # 20181006)
Gelatin-Separopore® 4B (SKU # 20181012)
Heparin-Separopore® 4B (SKU # 20181013)
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Protein A-Separopore® 4B-CL (SKU # 20181029)
Protein A-Separopore® 4B-CL (Lyophilized) (SKU # 20181028)
Protein A Blue-Separopore® 4B-MB (Macrobeads) (SKU # 20181042)
Protein A Red-Separopore® 4B-MB (Macrobeads) (SKU # 20181086)
View-IP™ Protein-A Red Separopore® 4B-CL (SKU # 20181041)
Protein G-Separopore® 4B-CL (SKU # 20181030)
Ubiquitin-Separopore® 4B (SKU # 20181040)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability.
The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography Application: .
The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligandand the sample.
Strptavidin-Separopore® 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
It can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Separopore from which the antigen can be eluted.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Native Streptavidin (from Streptomyces avidinii)
Matrix: Separopore 4B (agarose beads, 4%)
Particle size range: 53 -180 µm
Matrix activation: Cyanogen bromide
Ligand density: 2 mg streptavidin / ml drained gel
Binding capacity: >20 µg biotin / ml drained gel
Exclusion limit: ~ 2 x 107
pH stability: 3 – 10
Binding buffer: 50 mM ammonium carbonate, 0.5 M NaCl, pH 10
Elution buffer: 50 mM ammonium acetate, 0.5 M NaCl, pH 4
Supplied as suspension in buffered saline solution with 0.05% sodium azide.
Storage: 2 – 8 °C
References:
Ku protein as the main cellular target of cell-surface-bound circulating DNA. Expert Opin Biol Ther. (2012) 12: S35-41.
Purification of the proprotein convertase furin by affinity chromatography based on PC-specific inhibitors. Biol Chem. (2011) 392: 973-81.
Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: The example of the sarcoplasmic reticulum Ca(2+)-ATPase. Methods Mol Biol. (2010) 601: 247-67.
Binding characteristics of sperm with recombinant human zona pellucida glycoprotein-3 coated beads. Indian J Med Res. (2009) 130: 37-43.
The glutamic acid decarboxylase 65 immunoglobulin G subclass profile differs between adult-onset type 1 diabetes and latent autoimmune diabetes in adults (LADA) up to 3 years after clinical onset. Clin Exp Immunol. (2009) 157: 255-60.
Quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins. Methods Mol Biol. (2008) 418: 89-100.
Quantification of diphtheria toxin mediated ADP-ribosylation in a solid-phase assay. Clin Chem. (2007) 53: 1676-83.
Determination of glutamic acid decarboxylase antibodies (GADA) IgG subclasses - comparison of three immunoprecipitation assays (IPAs). Clin Exp Immunol. (2007) 150: 68-74.
Factor H is a dermatan sulfate-binding protein: identification of a dermatan sulfate-mediated protease that cleaves factor H. J Biochem. (2005) 137: 225-33.
In situ imaging and isolation of proteins using dsDNA oligonucleotides. Nucleic Acids Res. (2004) 32: e165.
Biotinylated tags for recovery and characterization of ribonucleoprotein complexes. Biotechniques. (2004) 37:608-10.
Purification of biotinylated proteins on streptavidin resin: a protocol for quantitative elution. Proteomics. (2004) 4: 2296-9.
The effect of free DNA on the interactions of the estrogen receptor bound to hormone, partial antagonist or pure antagonist with target DNA. Eur J Biochem. (1995) 231: 204-13.
Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes. Biotechnology (N Y). (1995) 13: 155-60.
Identification of a receptor candidate for the carboxyl-terminal cell binding domain of thrombospondins. J Biol Chem. (1994) 269: 29650-7.
Biotinylation of transducin and G0 from bovine brain. J Protein Chem. (1991) 10: 325-32.
Biotin-dependent expression of the asialoglycoprotein receptor in HepG2. J Biol Chem. (1988) 263: 11280-3.
Effect of plasma biotinyl-peptides on biotinidase activity. Biofactors. (1988) 1: 179-85.
Specific isolation of surface glycoproteins from intact cells by biotinylated concanavalin A and immobilized streptavidin. Anal Biochem. (1986) 156: 463-72.
Related products:
Avidin-Separopore® 6B (SKU # 20181060)
Calmodulin-Separopore® 4B (SKU # 20181001)
E. coli Protein-Separopore® 4B (SKU # 20181006)
Gelatin-Separopore® 4B (SKU # 20181012)
Heparin-Separopore® 4B (SKU # 20181013)
KLH-Separopore® 4B (SKU # 20181016)
Papain-Separopore® 4B (SKU #20181027)
Protein A-Separopore® 4B-CL (SKU # 20181029)
Protein A-Separopore® 4B-CL (Lyophilized) (SKU # 20181028)
Protein A Blue-Separopore® 4B-MB (Macrobeads) (SKU # 20181042)
Protein A Red-Separopore® 4B-MB (Macrobeads) (SKU # 20181086)
View-IP™ Protein-A Red Separopore® 4B-CL (SKU # 20181041)
Protein G-Separopore® 4B-CL (SKU # 20181030)
Ubiquitin-Separopore® 4B (SKU # 20181040)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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