SwiftX™ DNA

Rapid. Smart. Anywhere.
Discover the convenient DNA extraction protocols of SwiftX™ DNA

Intended purpose
SwiftX™ DNA is designed for rapid extraction of genomic and mitochondrial DNA from human or animal samples as well as for extraction of DNA from bacteria, parasites (protozoa as well as helminth eggs) and viruses.

SwiftX™ DNA was developed to match the workflow and performance of the discontinued QIAGEN SpeedXtract® Nucleic Acid Kit.Reverse Purification

Advantages
Reverse Purification
One of the two differentiators to most rapid DNA extraction kits is our Reverse Purification technology, which actively removes inhibitors and cell debris from the cell lysate containing the DNA. This is why our extraction protocols work well with much more sample types than direct extraction methods of competitors. Our Reverse Purification is performed using proprietary magnetic particles and does not need bulky or expensive equipment such as a centrifuge or addition of Chelex resin - a simple magnetic stand is sufficient

Cell capture
Our proprietary centrifugation-free cell capture technology is unique among nucleic acid extraction solutions available on the market. It can be utilized to concentrate human & animal cells, parasites, fungi and bacteria in liquid samples in just 4 minutes using nothing else than a magnetic rack. In a second step, the concentrated cells are resuspended in lysis buffer and DNA as well as RNA is extracted.

Rapid and flexible
SwiftX™ DNA is intended to be a powerful research tool, which can be applied to a multitude of specimens and applications. Thus, we offer 3 different protocols for using the kit. Each of the protocols is tailored to particular sample types that shall be processed. The direct extraction approach using protocol 1 for swabs and solid samples is as short as 7 minutes. Protocols 2 and 3 are intended to be used for liquid specimens and make use of our cell capture technology and can be performed in as little as 10 minutes. Additional use of Proteinase K improves lysis efficiency of skin, fungi and gram-positive bacteria.

Applicable extraction targets
- Human and animal cells
- Gram-positive and gram-negative bacteria
- Parasitic protozoa and helminths
- Fungi
- Viruses

Applicable sample types
- Solid samples: swabs, pre-concentrated cells, dried blood spots, FTA cards, tissue (skin, muscle, liver, brain, bone marrow), hair follicles, etc.
- Liquid samples (up to 1mL): urine, cell cultures, saliva, sputum, fine-needle aspirates, tissue fluid, cerebrospinal fluid, throat washes, whole blood, tissue suspensions, liquid-based cytology media, swab samples in transport media, etc.

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Protocols

Protocol 1
Direct extraction workflow incl. reverse purification:
- mix sample with Buffer DL and Beads A
- incubate for 5 to 15 minutes at 95°C
- remove tubes from heat block and place in a magnetic rack
- use supernatant for downstream application

To be used with the following sample types:
- Swabs (e.g. buccal, nasal, pharyngeal, rectal)
- Pre-concentrated cells
- Dried blood spots, blood cards
- Tissue samples (e.g. skin, muscle, liver, brain, bone marrow)
- Fine-needle aspirates
- Tissue fluid, cerebrospinal fluid
- Hair follicles, tongue scrapings


Protocols 2 and 3
Workflow for cell capture, extraction and reverse purification:
- mix sample with Buffer EN and Beads A
- incubate for 3 minutes at ambient temperature
- place tubes in a magnetic rack
- discard supernatant
- Protocol 3 only: wash Beads A once with Buffer EN
- resuspend Beads A in Buffer DL
- incubate for 5 to 15 minutes at 95°C
- remove tubes from heat block and place in a magnetic rack
- use supernatant for downstream application

To be used with the following sample types:
- Whole blood
- Urine
- Saliva, throat washes
- Homogenized tissue suspensions
- Liquid-based cytology medium
- Mammalian and bacterial cells in culture medium<

Handbook - SwiftX™ DNA
Product Information – SwiftX™ DNA
Product Sheet – SwiftX™ DNA
Technical Sheet – SwiftX™ DNA
Application Note 2021-02 – Extraction of DNA from human epithelial specimens in liquid-based cytology (LBC) media.
Application Note 2022-01 – Processing of large volumes of urine for extraction of DNA.
Application Note 2022-03 – Extraction of Leishmania DNA from lymph node aspirates and cell culture
Application Note 2023-01 – Extraction of genomic DNA from dried blood spots and FTA cards
YouTube Webinar: Fast nucleic acid extraction and point-of-care diagnostics
YouTybe Webinar: SwiftX rapid extraction technology
YouTube Webinar: Diagnosis of urogenital Schistosomiasis
YouTybe Webinar: Point-of-Care RNA & DNA extraction
YouTube Webinar: Diagnosis of Mycobacterium ulcerans from skin lesions