SwiftX™ Sepsis

Intended use
SwiftX™ Sepsis is intended to be used for extraction and purification of DNA of bacterial and fungal cells from up to 10 mL of whole blood. For research use only.
Extracted DNA samples can be applied to a wide range of analytical methods, such as spectrophotometric and fluorometric analyses, real-time quantitative PCR and isothermal amplification, and next-generation sequencing.

Principles of the method
SwiftX™ Sepsis is designed for extraction and purification of DNA from up to 10mL of venous whole blood. The different components of SwiftX™ Sepsis have specific functionalities within the workflow of DNA extraction.

Buffer FBC stabilizes biological cells such as bacteria, fungal cells, and white blood cells during cell capturing and enables efficient binding of biological cells to the paramagnetic particles Beads A.

Incubation with Buffer FBW facilitates the removal of contaminants, impurities and inhibitors.

Buffer FBL enables an efficient cell lysis and release of DNA. Optimal cell lysis is achieved by utilizing Proteinase K and applying a two-step heating regimen.

Beads A are paramagnetic particles with fast and strong magnetic response utilized for binding biological cells and for removal of cell debris and other particulate matter from the lysis mixture.

Content of the kit
- Buffer FBC
- Buffer FBW
- Buffer FBL
- Beads A
- Proteinase K