T4 DNA Ligase

T4 DNA Ligase is highly efficient, accurate and rapid enzyme designed for an efficient ligation of cohesive and blunt ended DNA insert into plasmid vectors in 5-15 minutes. It is based on the combination of T4 DNA Ligase with a Premium 5x Ligation Buffer. The ligation reaction mixture is used directly for bacterial transformation using conventional transformation procedures. It enables sticky end or blunt end DNA ligation in only 5 minutes at Room Temperature.

Advantages & Features
- Recombinant: isolated from E. coli.
- Highly efficient: 3,500 white colonies and 10 blue colonies expected under optimal conditions.
- Fast and easy protocol: get 24 % of efficiency at 5 minutes and 75 % at 15 minutes.
- Powerful: allows cloning insert amounts < 1 ng/kb.
- Versatile: enables sticky end or blunt end DNA Ligation.

Specifications
Unit Definition:
One Weiss unit of the enzyme catalyses the conversion of 1 nmol of [32PPi] into Norit- adsorbable form in 20 min at 37 ºC. One Weiss unit is equivalent to approximately 200 cohesive end ligation (CEL) units and one CEL unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of 1 μg lambda DNA in 30 min. at 16 ºC.

Includes
- 200 µL T4 DNA Ligase (5 U Weiss/µL)
- 1.5 mL T4 DNA Ligase Buffer (10x)

Applications
- Routine cloning procedure: blunt and cohesive double stranded DNA cloning.
- TA cloning.
- Joining of adaptors with blunt or cohesive ends.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C.