T4 DNA Polymerase 

Bacteriophage T4 DNA Polymerase is a DNA-directed 5’ to 3’ DNA polymerase. It is the product of gene 43 from bacteriophage T4 and is therefore often referred to as T4 gp43 DNA Polymerase. The enzyme catalyzes the polymerization of deoxynucleotide triphosphates in a 5’ to 3’ direction. It possesses very active 3’ to 5’ exonuclease activity that is more active on single than double stranded DNA, T4 DNA polymerase has no 5’ to 3’ exonuclease activity. For polymerase activity the enzyme requires DNA with a 5’ protruding end and a high concentration of TruePure™ dNTPs.

Advantages & Features
- Recombinant: isolated from a recombinant source (E.coli cells with a cloned gene 43 of bacteriophage T4).
- Powerful: stronger 3′-5′ exonuclease activity on single-stranded than on double-stranded DNA.
- Complete solution: supplied with 10x Reaction Buffer.

Specifications
Unit definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 °C using DNAse I-Nicked DNA as template-primer.

Includes
- T4 DNA Polymerase (5 U/μL)
- Reaction Buffer (5x)

Applications
- Generation of blunt double-stranded DNA from DNA containing 5′ overhangs.
- Generation of blunt double-stranded DNA from DNA containing 3′ overhangs.
- 5′-end or 3′-end labeling of double-stranded DNA.
- In vitro mutagenesis.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C.