T7 Biotin16 RNA Labeling Kit

T7 Biotin16 RNA Labeling Kit is designed to generate randomly biotin-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments.

Biotin-16-UTP (Biotin-16-uridine-5’-triphosphate) is a biologically active analog of UTP (uridine 5′-triphosphate) that is readily incorporated into RNA during an in vitro transcription reaction by RNA Polymerases such as phage T7 RNA Polymerase.

Since T7 RNA polymerase does not significantly discriminate between the natural and the biotinylated nucleotide, the number of biotinylated positions per RNA transcript can be controlled by adjusting the ratio of the biotinylated nucleoside triphosphate (NTP) to the naturally occurring NTP in the transcription reaction. The biotin-labeled RNA can then be detected by a reporter molecule attached to streptavidin, avidin, or ant-biotin antibody.

Advantages & Features
- High-Quality Kit: High enzymatic purity, specificity, and activity.
- Scaleable: Increase the reaction size to produce milligram amounts of RNA.
- Easy and time-saving protocol with minimal handling steps.
- Convenient: Rnase Inhibitor included.

Includes
- 3 x 40 μl T7 RNA Polymerase mix (include RNase Inhibitor)
- 200 μl T7 Reaction Buffer 10x (HEPES-based)
- 100 μl ATP (100 mM)
- 100 μl GTP (100 mM)
- 100 μl CTP (100 mM)
- 100 μl UTP (100 mM)
- 100 μl DTT (100 mM)
- 30 µl Biotin-16-UTP (10mM)
- 10 µl T7 Control template 1 (200 ng/µl)
- 1.2 ml Nuclease-Free Water

Applications
- in situ hybridization.
- Southern blots.
- Microarray hybridization.
- Chromosome mapping.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).