T7 Digoxigenin RNA Labeling Kit

T7 Digoxigenin RNA Labeling Kit is designed to generate randomly Digoxigenin-modified RNA probes by in vitrotranscription with Dioxigenin-11-UTP.

Digoxigenin (DIG) is a steroid hapten derived from plants of the genus Digitalis (D. lanata, and the purple foxglove D. purpurea). It is widely used for generating non-radioactive probes and is an alternative to biotinylation.

Like biotin, DIG-labeled probes support both chromogenic and chemiluminescence detection formats. RNA probes are synthesized by in vitro transcription with DIG-11-UTP. These nucleotides are linked via a spacer arm to DIG. The resulting DIG-labeled molecules then function as hybridization probes in much the same manner as any other type of probe.

Advantages & Features
- High-Quality Kit: High enzymatic purity, specificity, and activity.
- Scaleable: Increase the reaction size to produce milligram amounts of RNA.
- Easy and time-saving protocol with minimal handling steps.
- Convenient: Rnase Inhibitor included.

Includes
- 2x 40 μl T7 RNA Polymerase mix
- 200 μl T7 10X Reaction Buffer
- 100 μl ATP (100 mM)
- 100 μl GTP (100 mM)
- 100 μl CTP (100 mM)
- 100 μl UTP (100 mM)
- 25 μl DIG-11-UTP (10mM)
- 100 μl DTT (100 mM)
- 10 µl Control template 1 (200 ng/µl)
- 1.2 ml Nuclease-Free Water

Applications
- in situ hybridization.
- Southern blots.
- Microarray hybridization.
- Chromosome mapping.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).