T7 Fluorescein RNA Labeling Kit

T7 Fluorescein RNA Labeling Kit is designed to generate randomly fluorescein-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments.

The fluorescein labeled analog of uridine triphosphate (Fluorescein-12-UTP) is used for labeling RNA probes. Fluorescein-12-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP via in vitro transcription with RNA polymerases and optimized reaction buffer. fluorescein-modified RNA probe may be detected directly via fluorescence spectroscopy.

Advantages & Features
- Quality Kit: High enzymatic purity, specificity, and activity.
- Convenient: Rnase inhibitor included.
- Easy and time-saving protocol with minimal handling steps.

Includes
- 2 x 40 μl T7 RNA Polymerase mix (include RNase inhibitor)
- 200 μl T7 Reaction Buffer 10x (HEPES-based)
- 100 μl ATP (100 mM)
- 100 μl GTP (100 mM) 100 μl
- 100 μl CTP (100 mM) 100 μl
- 100 μl UTP (100 mM) 100 μl
- 10 μl Fluorescein-12-UTP (5mM)
- 100 μl DTT (100 mM)
- 10 µl Control template 1 (200 ng/µl)
- 1.2 ml Nuclease-Free Water

Applications
Fluorescein-labeled RNA is used for hybridization to:
- in situ hybridization to tissues.
- Southern blots.
- plaque or colony lifts.
- RNase protection experiments.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).