T7 RNA Polymerase

T7 RNA Polymerase is a recombinant protein from the bacteriophage T7 that is routinely used to produce RNA sequences from DNA templates. The protein shows highly stringent specificity for its own promoter sequence that besides its consistent yields makes it suitable for producing large amount of a designed RNA. This recombinant version is purified from E. coli.

GMP-grade is also available.

Advantages & Features
- Isolated form a recombinant source.
- High-Purity: ≥ 95% determined by SDS.
- Extensivelly quality controlled: Endo/exonucleases, non-specific DNAse and RNAses free, Purity and Promoter Specificity.
- GMP-grade available.

Specifications
Protein information:
- Predicted MW: 98,855 kDa
- Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie® blue staining.
- Buffer: 10X Reaction Buffer: 40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine (pH 7.9 @ 25°C).
- Biological activity: One unit is defined as the amount of enzyme required to generate 1 nmol RNA in 1 hour at 37°C determined by a fluorimetric method.

Gene information:
- Synonyms: T7RNAP
- Official Symbol: T7p07
- Specie: Escherichia phage T7
- GenBank accession number: NC_001604.1
- NCBI Protein RefSeq: NP_041960.1
- Protein Family: Family of single-subunit RNAPs that includes the phage RNAPs (T3,K11, SP6, N4, and others) as well as the mitochondrial RNAPs.

Includes
- 5,000 U T7 RNA Polymerase (50 U/µL)
- T7 RNA Polymerase Reaction Buffer (1mL)

Applications
- Synthesis of mRNA, RNA standard template for RT-PCR, RNA probes for hybridization.
- Preparation of RNA vaccines.
- Studies of RNA secondary structure and RNA-protein interactions, RNA splicing.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).