Taq LA PCR Master Mix
€0.00
In stock
SKU
VN170MC
Catalog Number: VN170MC
Size: 500 x 25 µl rxns
Other Size(s): 250 x 25 µl rxns
Datasheet
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Size: 500 x 25 µl rxns
Other Size(s): 250 x 25 µl rxns
Datasheet
Request Information
Taq & KlenTaq Pols and Master Mixes (6)
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3 DNA polymerase activity and a 5´→3´ exonuclease activity. Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
KlenTaq® is a Klenow-fragment analog of Taq DNA polymerase. It is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity, robust and thermostability.
KlenTaq-S is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability, plus an extra mutation rendering the enzyme PAP function (Pyrophosphorolysis-activated polymerization). The enzyme is capable of detecting rare mutations, large heterozygous deletions, gene duplications, etc. in the presence of a large excess of wild type allele and inorganic pyrophosphate). In theory PAP can detect a single base mutation in 3x1011copies of wild type allele.
Taq and Klentaq LA® (Long Accurate) versions of the enzymes are mixtures of Taq / KlenTaq and a proof reading enzyme. They can be used to amplify a long gene target with high fidelity.
Taq or Taq LA Mix is a ready-to-use 2X mixture of Taq or Taq LA DNA polymerase, salts, magnesium and dNTPs for setting up a trouble-free PCR reaction. All you have to do is to add template, specific primers and water, thereby save time, effort and minimize pipetting error.
2X Taq or Taq LA PCR Mix is recommended for all standard PCR applications. The mix is comprised of Taq DNA polymerase or Taq DNA polymerase LA DNA polymerase (for amplification of a longer fragment) and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. It has been developed to give more robust amplification than other commonly-used mixes, allowing it to perform well with challenging templates.
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3 DNA polymerase activity and a 5´→3´ exonuclease activity. Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
KlenTaq® is a Klenow-fragment analog of Taq DNA polymerase. It is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity, robust and thermostability.
KlenTaq-S is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability, plus an extra mutation rendering the enzyme PAP function (Pyrophosphorolysis-activated polymerization). The enzyme is capable of detecting rare mutations, large heterozygous deletions, gene duplications, etc. in the presence of a large excess of wild type allele and inorganic pyrophosphate). In theory PAP can detect a single base mutation in 3x1011copies of wild type allele.
Taq and Klentaq LA® (Long Accurate) versions of the enzymes are mixtures of Taq / KlenTaq and a proof reading enzyme. They can be used to amplify a long gene target with high fidelity.
Taq or Taq LA Mix is a ready-to-use 2X mixture of Taq or Taq LA DNA polymerase, salts, magnesium and dNTPs for setting up a trouble-free PCR reaction. All you have to do is to add template, specific primers and water, thereby save time, effort and minimize pipetting error.
2X Taq or Taq LA PCR Mix is recommended for all standard PCR applications. The mix is comprised of Taq DNA polymerase or Taq DNA polymerase LA DNA polymerase (for amplification of a longer fragment) and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. It has been developed to give more robust amplification than other commonly-used mixes, allowing it to perform well with challenging templates.
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