TBP Polyclonal Antibody

Background:
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
GTF2D, GTF2D1, HDL4, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, TATA-box-binding protein, TBP, TBP, TFIID, Transcription initiation factor TFIID TBP subunit

Immunogen: Synthetic peptide of Human TATA-box-binding protein

Swissprot: P20226
Gene ID: 6908

Calculated MW: 38 kDa
Observed MW: 38 kDa

Purification Method: Antigen Affinity Purification

Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3.

Concentration: 1mg/mL
Dilution: WB 1:4000-1:6000

Storage:
Store at -20℃. Avoid freeze / thaw cycles.