TE (pH 7.4)

Tris-EDTA (TE) is a high quality, reliable and convenient solution which incorporates a buffer, Tris and a chelanting agent, EDTA. EDTA avoids the degradation of DNA and RNA chelating magnesium or other divalent metal ions.

Generally, TE is used to solubilize DNA and RNA, protecting it from degradation. Moreover, in the fixation with immunohistochemichal, formalin and other aldehydes, fixation generates protein cross-links that mask the antigenic sites, giving weak positives or false negatives. With TE buffer the protein cross-links are broken, antigens and epitopes are unmasked therefore enhancing the intensity of staining of antibodies.

GMP-grade is also available.

Advantages & Features
- Reliable: rigorous quality control standards to guarantee lot-to-lot consistency.
- High Quality: free of DNase, RNase or protease activities.
- Time-saving due its ready-to-use format that saves experiment preparation time.
- Complete solution: includes chelating agent.
- Versatile: pH adjusted for DNA/RNA work.
- Sterile: by autoclaving or filtration.

Specifications
- Chemicals: Analytical grade.
- Composition: 0.1 M Tris-HCl, 0.010 M EDTA (10x).
- pH: 7.4 ± 0.05 at 25 ºC.

Includes
1,000 mL TE Ready-to-use Solution (10x)

Applications
- Running buffer and gels for RNA analysis native and denaturing.
- Polyacrylamide and agarose gels.
- Nucleic acid electrophoresis.
- Transfer buffer in Northern Blotting.

Shipping & Storage
Shipped in: Ambient Temperature.
Storage: Room Temperature.