TNAP (clone ), anti-human, mouse, rat

Background:
Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoproteins that catalyze the hydrolysis of phospho-monoesters into an inorganic phosphate and an alcohol. There are at least four distinct but related alkaline phosphatases: intestinal (IAP), placental (PLAP), placental-like (ALP-1 or GCAP) and tissue non-specific (TNAP). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. TNAP is widely expressed in liver, kidney, bone, stomach and colon, and is therefore referred to as the tissue non-specific form of AP. TNAP, in conjuntion with plasma cell membrane glycoprotein-1, function in bone mineralization; however, mice that lack a functional form of TNAP show normal skeletal development.

Alternative Name:
Alkaline phosphatase, tissue-nonspecific isozyme, AP-TNAP, TNSALP, Alkaline phosphatase liver/bone/kidney isozyme, ALPL

Application Dilution: WB: 1:1000-1:5000, IHC/ICC: 1:50-1:200, IP: 1:50-1:200, FC: 1:10-1:100

Specificity: This antibody detects endogenous levels of TNAP and does not cross-react with related proteins.

Immunogen:
Recombinant antibody.

MW: ~ 57 kDa

Swis Prot.: P05186

Purification & Purity:
Protein A affinity purified

Format:
Recombinant Rabbit Monoclonal Antibody. 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.