Toscana virus RNA PCR Kit CE/IVD
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SKU
RealCycler TOSC-
Catalog Number: RealCycler TOSC-
1 Intended use:
RealCycler TOSC is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of RNA from Toscana virus in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Toscana virus is detected in the corresponding channel of FAM fluorophore and the internal control is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity: Toscana virus: 1 copy/μL.
The analytical sensitivity has been determined by limit dilution. This sensitivity has been showed in repeated assays with reproducibility over 95%.
Specificity: Toscana virus: nucleoprotein gene.
Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).
4. Contents
RealCycler TOSC includes the AmpliMix, a retrotranscriptase and a Toscana RNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
RealCycler TOSC is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of RNA from Toscana virus in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Toscana virus is detected in the corresponding channel of FAM fluorophore and the internal control is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity: Toscana virus: 1 copy/μL.
The analytical sensitivity has been determined by limit dilution. This sensitivity has been showed in repeated assays with reproducibility over 95%.
Specificity: Toscana virus: nucleoprotein gene.
Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).
4. Contents
RealCycler TOSC includes the AmpliMix, a retrotranscriptase and a Toscana RNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
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