Tosyl Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181039
Application: Tosyl-activated Separopore® 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated Separopore® 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through reduced disulfide bounds in the hinge region is a popular way to couple without interfering with the antigen binding region.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Matrix active group: p-toluenesulfonyl chloride (tosyl chloride)
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 μm
Groups coupled to: Primary amines at pH range 9 – 10
Phosphate, HEPES, bicarbonate and borate buffers should be used
Binding capacity: 4 – 8 mg chymotrypsin /ml matrix
Storage: 2 – 8 °C
References:
Lysozyme immobilized on micro-sized magnetic particles: kinetic parameters at wine pH. Appl Biochem Biotechnol. (2012) 166: 1736-46.
Production and evaluation of antibodies and phage display-derived peptide ligands for immunomagnetic separation of Mycobacterium bovis. J Clin Microbiol. (2012) 50: 1598-605.
Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor. Anal Bioanal Chem. (2012) 403: 939-46.
Magneto immunosensor for gliadin detection in gluten-free foodstuff: towards food safety for celiac patients. Biosens Bioelectron. (2011) 27: 46-52.
Quantification of the electrophilic reactivities of aldehydes, imines, and enones. J Am Chem Soc. (2011) 133: 8240-51.
Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensors. Biosens Bioelectron. (2009) 24: 2057-63.
Selective benzylic and allylic alkylation of protic nucleophiles with sulfonamides through double Lewis acid catalyzed cleavage of sp3 carbon-nitrogen bonds. Chemistry. (2009) 15: 793-7.
Electrochemical magneto immunosensing of antibiotic residues in milk. Biosens Bioelectron. (2007) 22: 2184-91.
Practical synthesis of trans-tert-butyl-2-aminocyclopentylcarbamate and resolution of enantiomers. J Org Chem. (2006) 71: 8655-7.
Electrochemical magnetoimmunosensing strategy for the detection of pesticides residues. Anal Chem. (2006) 78: 1780-8.
Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods. Biotechniques. (2002) 32:178-80, 182-3.
A colorimetric detection system for Calymmatobacterium granulomatis. Sex Transm Infect. (2000) 76: 134-6.
A role for scavenger receptors in phagocytosis of protein-coated particles in rainbow trout head kidney macrophages. Dev Comp Immunol. (1998) 22: 533-49.
Particle electrophoresis of micrometric-sized superparamagnetic particles designed for magnetic purification of cells. Electrophoresis. (1998) 19: 2485-90.
The IgV domain of human B7-2 (CD86) is sufficient to co-stimulate T lymphocytes and induce cytokine secretion. Int Immunol. (1997) 9: 805-13.
Isolation and pure culture of microvascular endothelial cells from the fetal skin. Yonsei Med J. (1996) 37: 186-93.
Steady-state force-velocity relation in the ATP-dependent sliding movement of myosin-coated beads on actin cables in vitro studied with a centrifuge microscope. Proc Natl Acad Sci U S A. (1990) 87: 7893-7.
Removal of HIV antigens and HIV-infected cells in vitro using immunomagnetic beads. J Virol Methods. (1990) 29: 23-31.
Binding of human endothelium to Ulex europaeus I-coated Dynabeads: application to the isolation of microvascular endothelium. J Cell Sci. (1990) 96: 257-62.
Related products:
CDI-activated Separopore® 4B (SKU # 20181002)
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 4B (SKU # 20181003)
CNBr-activated Separopore® 6B (SKU # 20181062)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
NHS-activated Separopore® 4B-CL (SKU # 20181025)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Matrix active group: p-toluenesulfonyl chloride (tosyl chloride)
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 μm
Groups coupled to: Primary amines at pH range 9 – 10
Phosphate, HEPES, bicarbonate and borate buffers should be used
Binding capacity: 4 – 8 mg chymotrypsin /ml matrix
Storage: 2 – 8 °C
References:
Lysozyme immobilized on micro-sized magnetic particles: kinetic parameters at wine pH. Appl Biochem Biotechnol. (2012) 166: 1736-46.
Production and evaluation of antibodies and phage display-derived peptide ligands for immunomagnetic separation of Mycobacterium bovis. J Clin Microbiol. (2012) 50: 1598-605.
Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor. Anal Bioanal Chem. (2012) 403: 939-46.
Magneto immunosensor for gliadin detection in gluten-free foodstuff: towards food safety for celiac patients. Biosens Bioelectron. (2011) 27: 46-52.
Quantification of the electrophilic reactivities of aldehydes, imines, and enones. J Am Chem Soc. (2011) 133: 8240-51.
Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensors. Biosens Bioelectron. (2009) 24: 2057-63.
Selective benzylic and allylic alkylation of protic nucleophiles with sulfonamides through double Lewis acid catalyzed cleavage of sp3 carbon-nitrogen bonds. Chemistry. (2009) 15: 793-7.
Electrochemical magneto immunosensing of antibiotic residues in milk. Biosens Bioelectron. (2007) 22: 2184-91.
Practical synthesis of trans-tert-butyl-2-aminocyclopentylcarbamate and resolution of enantiomers. J Org Chem. (2006) 71: 8655-7.
Electrochemical magnetoimmunosensing strategy for the detection of pesticides residues. Anal Chem. (2006) 78: 1780-8.
Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods. Biotechniques. (2002) 32:178-80, 182-3.
A colorimetric detection system for Calymmatobacterium granulomatis. Sex Transm Infect. (2000) 76: 134-6.
A role for scavenger receptors in phagocytosis of protein-coated particles in rainbow trout head kidney macrophages. Dev Comp Immunol. (1998) 22: 533-49.
Particle electrophoresis of micrometric-sized superparamagnetic particles designed for magnetic purification of cells. Electrophoresis. (1998) 19: 2485-90.
The IgV domain of human B7-2 (CD86) is sufficient to co-stimulate T lymphocytes and induce cytokine secretion. Int Immunol. (1997) 9: 805-13.
Isolation and pure culture of microvascular endothelial cells from the fetal skin. Yonsei Med J. (1996) 37: 186-93.
Steady-state force-velocity relation in the ATP-dependent sliding movement of myosin-coated beads on actin cables in vitro studied with a centrifuge microscope. Proc Natl Acad Sci U S A. (1990) 87: 7893-7.
Removal of HIV antigens and HIV-infected cells in vitro using immunomagnetic beads. J Virol Methods. (1990) 29: 23-31.
Binding of human endothelium to Ulex europaeus I-coated Dynabeads: application to the isolation of microvascular endothelium. J Cell Sci. (1990) 96: 257-62.
Related products:
CDI-activated Separopore® 4B (SKU # 20181002)
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 4B (SKU # 20181003)
CNBr-activated Separopore® 6B (SKU # 20181062)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
NHS-activated Separopore® 4B-CL (SKU # 20181025)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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