Toxoplasma gondii PCR Kit RUO

Toxoplasma gondii PCR Kit RUO

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In stock
SKU
RealCycler TGON-
Catalog Number: RealCycler TGON-

1 Intended use:
RealCycler TGON is a diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from Toxoplasma gondii in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.

2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).

The amplification of Toxoplasma gondii is detected in the corresponding channel of FAM fluorophore and CHIC is detected in the corresponding channel of Alx532 or HEX fluorophore

3 Technical specifications
Sensitivity: 10 copies/μL
The analytical sensitivity has been determined by limit dilution. This sensitivity has been showed in repeated assays with reproducibility over 95%.

These technical specifications have been verified usin gQCMD 2012 Toxoplasma gondii panel

Specificity: Toxoplasma gondii: Repetitive 529 bp DNA fragment..

Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).

4. Contents
RealCycler TGON-U includes the AmpliMix and a T. gondii DNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.

Sample material: Purified DNA from clinical samples.
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