Tris-Glycine Buffer (pH 8.3)

Tris-Glycine Buffer (TG) is a high quality and convenient running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.

It is also used to make Tris-glycine/20% methanol Western transfer buffer, which is the most frequently used protein transfer buffer for wet blot transfers.

GMP-grade is also available.

Advantages & Features
- Reliable: rigorous quality control standards to guarantee lot-to-lot consistency.
- High Quality: free of DNAse, RNase or protease contamination.
- Proven performance: for protein electrophoresis.
- Consistency guaranteed.

Specifications
- Chemicals: Analytical grade.
- Composition: 0.025 M Tris, 0.192 M glycine.
- pH: 8.3 ± 0.2 at 25 °C.

Includes
1,000 mL of Tris-Glycine Buffer Aqueous solution format

Applications
- Protein Electrophoresis.
- Denatured protein Electrophoresis.
- Polyacrylamide gel Electrophoresis.
- Western Blotting.

Shipping & Storage
Shipped at: Room temperature.
Storage: Room temperature.