Tropheryma whipplei PCR Kit CE/IVD
€0.00
In stock
SKU
RealCycler TRWH-
Catalog Number: RealCycler TRWH-
1 Intended use:
RealCycler TRWH is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from Tropheryma whipplei in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Tropheryma whipplei is detected in the corresponding channel of FAM fluorophore and the internal control is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity: 10 copies/μL .
Specificity: Tropheryma whipplei (WiSP family protein).
4 Contents
RealCycler TRWH includes the AmpliMix and a T. whipplei DNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
RealCycler TRWH is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from Tropheryma whipplei in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Tropheryma whipplei is detected in the corresponding channel of FAM fluorophore and the internal control is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity: 10 copies/μL .
Specificity: Tropheryma whipplei (WiSP family protein).
4 Contents
RealCycler TRWH includes the AmpliMix and a T. whipplei DNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
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