TRPM7 (Phospho Ser-1513) Blocking Peptide
€155.00
In stock
SKU
ECM-TX5675
Background:
The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.
Background References
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23.
Sequence: Phospho-TRPM7 (Ser-1513) synthetic peptide corresponding to amino acids surrounding Ser-1513 in human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.
Specificity: The peptide is specifically recognized by TRPM7 (Ser-1513), antibody (TP5671) in ELISA, and has been shown to block the reactivity of TP5671 in Western blot. In addition, the peptide is recommended for use in blocking TP5671 reactivity in immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.
Background References
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23.
Sequence: Phospho-TRPM7 (Ser-1513) synthetic peptide corresponding to amino acids surrounding Ser-1513 in human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.
Specificity: The peptide is specifically recognized by TRPM7 (Ser-1513), antibody (TP5671) in ELISA, and has been shown to block the reactivity of TP5671 in Western blot. In addition, the peptide is recommended for use in blocking TP5671 reactivity in immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
| Is Featured? | No |
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