UACA/Nucling (clone AE-5), anti-human, mouse

Source:
A BALB/c mouse was immunized with nuclei of myeloid leukemia biopsy cells.
Fusion partner: NS-1.

Specifications:
UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease,and in patients with Graves' disease. UACA was also later identified as Nucling, a mRNA differentially expressed in F9 embryonal carcinoma cells, and that is up-regulatedduringcardiac muscle differentiation.UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.

Species reactivity:
Positive: human, mouse.

Applications:
Demonstrate UACA in human and murine cells by flow cytometry, immunofluorescence and immunoblot.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Western blot: +

Positive control: HeLa or 293T cells. Highly expressed in skeletal muscle, heart, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells.

References:
- Yamada, K., et al. Biochem. Biophys. Res. Commun.280: 1169-1176 (2001).
- Ohkura, T., et al. Biochem. Biophys. Res. Commun. 321: 432-440 (2004)