Ubiquitin Separopore® 4B
€0.00
In stock
SKU
BW-20181040
Application:
Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in intracellular protein degradation, calpain and lysosomal proteases, proteomics, and ubiquitination analysis. Ubiquitin- Separopore® 4B has been used in a study to produce evidence for a particulate location of ubiquitin conjugates and ubiquitin-conjugating enzymes in the rabbit brain. Ubiquitin- Separopore® 4B has also been used to study fertilization of the ascidian, Halocynthia roretzi. Ubiquitin is covalently linked to Separopore® beads via primary amine coupling leaving the C-terminus free.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Ubiquitin (bovine)
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size: 52-165 µm
Matrix spacer: 6 carbon
Ligand density: 7 – 15 mg ubiquitin / ml drained gel
Binding Capacity: 2 mg protein /ml drained gel
Storage: 4 °C
Supplied as suspension in 1M NaCl containing 0.02% thimerosal
References:
Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA. (2006) 12: 292-302.
Conjugation of phenylalanine hydroxylase with polyubiquitin chains catalysed by rat liver enzymes. Biochim Biophys Acta. (2001) 1547: 379-86.
The ubiquityl-calmodulin synthetase system from rabbit reticulocytes: isolation of the ubiquitin-binding first component, a ubiquitin-activating enzyme. Eur J Biochem. (1998) 255: 482-91.
Induction of ubiquitin conjugating enzyme activity for degradation of topoisomerase II alpha during adenovirus E1A-induced apoptosis. Biochem Biophys Res Commun. (1997) 239: 823-9.
Affinity purification and characterization of protein gene product 9.5 (PGP9.5) from retina. Biochem J. (1996) 318: 711-6.
Demonstration of a ubiquitin binding site on murine haemopoietic progenitor cells: implication of ubiquitin in homing and adhesion. Br J Haematol. (1993) 84: 212-8.
Bovine lens epithelial cells have a ubiquitin-dependent proteolysis system. Biochim Biophys Acta. (1993) 1175: 181-7.
A ubiquityl-calmodulin synthetase that effectively recognizes the Ca(2+)-free form of calmodulin. FEBS Lett. (1993) 315: 347-52.
Purification and partial characterization of ubiquitin-activating enzyme from Saccharomyces cerevisiae. FEBS Lett. (1991) 289: 54-8.
The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6. EMBO J. (1990) 9: 1431-5.
Multiple (alpha-NH-ubiquitin)protein endoproteases in cells. J Biol Chem. (1989) 264: 10637-42.
Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown. J Biol Chem. (1983) 258: 8206-14.
Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins. J Biol Chem. (1982) 257: 13964-70.
Covalent affinity purification of ubiquitin-activating enzyme. J Biol Chem. (1982) 257: 2537-42.
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Streptavidin-Separopore® 4B (SKU # 20181037)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in intracellular protein degradation, calpain and lysosomal proteases, proteomics, and ubiquitination analysis. Ubiquitin- Separopore® 4B has been used in a study to produce evidence for a particulate location of ubiquitin conjugates and ubiquitin-conjugating enzymes in the rabbit brain. Ubiquitin- Separopore® 4B has also been used to study fertilization of the ascidian, Halocynthia roretzi. Ubiquitin is covalently linked to Separopore® beads via primary amine coupling leaving the C-terminus free.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Ubiquitin (bovine)
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size: 52-165 µm
Matrix spacer: 6 carbon
Ligand density: 7 – 15 mg ubiquitin / ml drained gel
Binding Capacity: 2 mg protein /ml drained gel
Storage: 4 °C
Supplied as suspension in 1M NaCl containing 0.02% thimerosal
References:
Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA. (2006) 12: 292-302.
Conjugation of phenylalanine hydroxylase with polyubiquitin chains catalysed by rat liver enzymes. Biochim Biophys Acta. (2001) 1547: 379-86.
The ubiquityl-calmodulin synthetase system from rabbit reticulocytes: isolation of the ubiquitin-binding first component, a ubiquitin-activating enzyme. Eur J Biochem. (1998) 255: 482-91.
Induction of ubiquitin conjugating enzyme activity for degradation of topoisomerase II alpha during adenovirus E1A-induced apoptosis. Biochem Biophys Res Commun. (1997) 239: 823-9.
Affinity purification and characterization of protein gene product 9.5 (PGP9.5) from retina. Biochem J. (1996) 318: 711-6.
Demonstration of a ubiquitin binding site on murine haemopoietic progenitor cells: implication of ubiquitin in homing and adhesion. Br J Haematol. (1993) 84: 212-8.
Bovine lens epithelial cells have a ubiquitin-dependent proteolysis system. Biochim Biophys Acta. (1993) 1175: 181-7.
A ubiquityl-calmodulin synthetase that effectively recognizes the Ca(2+)-free form of calmodulin. FEBS Lett. (1993) 315: 347-52.
Purification and partial characterization of ubiquitin-activating enzyme from Saccharomyces cerevisiae. FEBS Lett. (1991) 289: 54-8.
The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6. EMBO J. (1990) 9: 1431-5.
Multiple (alpha-NH-ubiquitin)protein endoproteases in cells. J Biol Chem. (1989) 264: 10637-42.
Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown. J Biol Chem. (1983) 258: 8206-14.
Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins. J Biol Chem. (1982) 257: 13964-70.
Covalent affinity purification of ubiquitin-activating enzyme. J Biol Chem. (1982) 257: 2537-42.
Related products:
Avidin-Separopore® 6B (SKU # 20181060)
Calmodulin-Separopore® 4B (SKU # 20181001)
E. coli Protein-Separopore® 4B (SKU # 20181006)
Gelatin-Separopore® 4B (SKU # 20181012)
Heparin-Separopore® 4B (SKU # 20181013)
KLH-Separopore® 4B (SKU # 20181016)
Papain-Separopore® 4B (SKU #20181027)
Protein A-Separopore® 4B-CL (SKU # 20181029)
Protein A-Separopore® 4B-CL (Lyophilized) (SKU # 20181028)
Protein A Blue-Separopore® 4B-MB (Macrobeads) (SKU # 20181042)
Protein A Red-Separopore® 4B-MB (Macrobeads) (SKU # 20181086)
View-IP™ Protein-A Red Separopore® 4B-CL (SKU # 20181041)
Protein G-Separopore® 4B-CL (SKU # 20181030)
Streptavidin-Separopore® 4B (SKU # 20181037)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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