ViRNAEx™ - Viral RNA Extractor

• CE IVD certified
• Fast and reliable viral RNA extraction using thermoblock
• Single step process – takes approx. 15 minutes
• No centrifugation needed
• No additional chemicals needed
• No additional equipment needed
• Cheapest extraction price worldwide
• Package 10 mL (100 rxns) or 50 mL (500 rxns)
• Instant procedure delivering RNA in minutes
• Ready-to-use RNA for high performance in any downstream application
• Uncomplicated handling
• Environmentally friendly

ViRNAEx™ provides fast purification of high-quality RNA from cells collected by standard nasopharyngeal swabbing. Samples can be conveniently delivered in classical viral transport media. A nucleic acid extract that is suitable for this PCR should be used with ViRNAEx™. Acceptable specimen types include nucleic acid extracts of nasal swabs, throat swabs, nasopharyngeal swabs, nasopharyngeal aspirate, tracheal aspirate, bronchial washing or bronchoalveolar washing.
RNA purification using the ViRNAEx™ can be automated on any pipetting automat. For smaller and larger samples, the volume of extracting buffer may be adjusted. The recommended starting volume is 100 µl of sampling solution.

Introduction
ViRNAEx™ RNA extraction solution enables instant and inexpensive RNA isolation from viruses with a possibility to use RNA subsequently in real time- PCR testing (Volume recommended: 5-10 µl of solution per qPCR reaction). The duration of RNA sample preparation is shortened on approx. 15 minutes including pipetting steps.

Recommended RT-PCR kit

Using CoVirED RT-PCR test for COVID-19 detection in combination with ViRNAEx™ is strongly recommended. CoVirED PCR protocol includes internal PCR reaction control as well as control of human cells presence using human endogenous control gene probe. CoVirED (Corona Virus Extraction and Detection kit) is delivered for a favorable price if combined with ViRNAEx™ (one package). More info here.

Package:
ViRNAEx™ – 10 mL (for 100 rxns)
ViRNAEx™ – 50 mL (for 500 rxns)

Product Specifications:
Storage after opening     : under 8 °C
Unit Definition                  : 10 mL or 50 mL
Quality Control                 : QAM 20200414
RNAse testing                  : RNAses free 20200414

CE IVD

ViRNAEx™ For fresh samples, only!!! Do not use VTM- frozen samples!!!

Protocol – Isolation of RNA from nasopharyngeal, buccal swabs

1. Mix 100 µl of the biological sample (VTM) with 100 µl of the extraction solution ViRNAEx™
2. Incubate at 70°C (158°F) for 11 or 15 minutes (Some VTMs may work better after heating for 15 minutes, please check your VTM for 11 or 15 minutes and adjust the best time for your samples)
3. Dilute the pre-heated mix 1:1 with nuclease free water
4. Use the diluted solution (5-10 µl) for down-stream PCR application