X-Gal

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) is a chromogenic substrate for beta-galactosidase.

β-Galactosidase cleaves the substrate and releases colorless galactose and 5-Bromo-4-chloro-3-indoxyl (X). The indoxyl will be oxidized to the insoluble 5,5′-dibromo-4,4′-dichloro-indigo, forming an intense blue precipitate.

Advantages & Features
- Purity: > 99% confirmed by HPLC.
- High Quality.
- Powder format.

Specifications
- CAS Number: 7240-90-6
- Chemical Formula: C14H15BrClNO6
- Molecular Weight: 408.63
- Assay (HPLC) > 98% w/w
- Purity (HPLC): > 99%
- Purity (TLC): single spot
- Water content (Karl Fischer): <1%
- Identity (IR): conforms to structure
- Solubility (5% w/v, DMF): soluble

Includes
1 g X-Gal

Applications
- Blue/white screening.
- Gene expression detection of β-galactosidase reporter.
- Detection of β-galactosidase activity in immunological and histochemical applications.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 ºC (NON Frost-Free Freezer).