XTT Cell Proliferation Assay Kit

XTT Cell Proliferation Assay Kit is an optimized, accurate and sensitive colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT (sodium 2,3,-bis(2methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium) is reduced to a highly colored formazan dye by dehydrogenase enzymes in metabolically active cells.

This conversion only occurs in viable cells and thus, the amount of the formazan produced is proportional to viable cells in the sample. The formazan dye formed in the assay is soluble in aqueous solution and quantified by measuring the absorbance at wavelength 450 nm using a spectrophotometer. An electron coupling reagent, such as PMS (N-Methylphenazonium methyl sulphate), can significantly improve the efficiency of XTT reduction in cells.

Advantages & Features
- Accurate: dye absorbance is proportional to the number of cells in each well.
- Sensitive: assayed even in low cell concentrations.
- Fast protocol: results within 2-5 hours with minimal handling steps.
- Time-saving protocol: avoids solubilisation step.
- Complete solution: includes all reagents needed for cell washing procedures.
- Safe: avoids radioactivity.
- Optimized: for high throughput assays (no washing or other steps that can cause cell loss and variability).
- Cost avoidance: allows performance directly in a microtiter plate.

Includes
- 2 x 25 mL XTT Cell Proliferation Assay Kit Reagent
- 1 mL Activation Reagent

Applications
- Spectrophotometric quantification of cell proliferation and viability in response to pharmaceutical, chemical, nutrients and environmental compounds.
- High Throughput Screening.

Shipping & Storage
Shipped at: Gel Pack.
Storage: -20 °C.
Recommendations: Protect XTT solution from light.