Yellow Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181098
Application:
Dye affinity chromatography is a protein purification procedure based on the high affinity of immobilized dyes for the binding sites on many proteins. It is a rapid, inexpensive, and versatile method that is applicable to the purification of crude cellular extracts.
Reactive Yellow 3-Separopore® 4B-CL is used in affinity chromatography, protein chromatography and dye resins. Reactive Yellow 3-Separopore® 4B-CL has been used in immunohistochemical localization studies to show where tyramine N-(hydroxycinnamoyl)transferase (THT) polypeptides occur in opium poppy. Reactive Yellow-Separopore® 4B-CL has also been used to purify human cholesteryl ester transfer protein.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Reactive Yellow 3
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size: 52 – 165 µm
Ligand density: 1 – 4 mg Reactive yellow 3 / ml drained gel
Binding capacity: ~500 units (citrate synthase)/ml drained gel
pH stability: 3 – 13
Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
Storage temp: 2 – 8 °C
References:
Purification, characterization, and immunolocalization of hydroxycinnamoyl-CoA: tyramine N-(hydroxycinnamoyl)transferase from opium poppy. Planta. (1999) 209: 33-44.
A new method for purification of human plasma retinol-binding protein and transthyretin. Biotechnol Appl Biochem. (2003) 38: 19-24.
Triazine dyes as inhibitors and affinity ligands of glycosyltransferases. Glycoconj J. (1999) 16: 719-23.
Well defined dye adsorbents for protein purification. J Mol Recognit. (1996) 9: 728-32.
Purification of human cholesteryl ester transfer protein by affinity chromatography on immobilized triazine dyes. Protein Expr Purif. (1994) 5: 285-90.
Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation. J Chromatogr. (1992) 584: 93-9.
Purification and characterization of vitamin B6-phosphate phosphatase from human erythrocytes. J Biol Chem. (1992) 267: 15978-83.
Dye-affinity purification of transthyretin from an unexploited by-product of human plasma chromatographic fractionation. J Chromatogr. (1992) 576: 87-93.
Identification and purification of a human Sertoli cell-secreted protein (hSCSP-80) stimulating Leydig cell steroid biosynthesis. J Clin Endocrinol Metab. (1991) 72: 1332-9.
Oestradiol-17 alpha dehydrogenase from chicken liver. Biochem J. (1984) 222: 761-8.
The interaction of yeast hexokinase with Procion Green H-4G. Biochem J. (1981) 197: 203-11.
Related products:
Red-Separopore® 4B-CL (SKU # 20181095)
Blue-Separopore® 4B-CL (SKU # 20181096)
Reactive Blue 4-Separopore® 4B-CL (SKU #20181097)
Green-Separopore® 4B-CL (SKU # 20181099)
Brown-Separopore® 4B-CL (SKU # 20181100)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Dye affinity chromatography is a protein purification procedure based on the high affinity of immobilized dyes for the binding sites on many proteins. It is a rapid, inexpensive, and versatile method that is applicable to the purification of crude cellular extracts.
Reactive Yellow 3-Separopore® 4B-CL is used in affinity chromatography, protein chromatography and dye resins. Reactive Yellow 3-Separopore® 4B-CL has been used in immunohistochemical localization studies to show where tyramine N-(hydroxycinnamoyl)transferase (THT) polypeptides occur in opium poppy. Reactive Yellow-Separopore® 4B-CL has also been used to purify human cholesteryl ester transfer protein.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Reactive Yellow 3
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size: 52 – 165 µm
Ligand density: 1 – 4 mg Reactive yellow 3 / ml drained gel
Binding capacity: ~500 units (citrate synthase)/ml drained gel
pH stability: 3 – 13
Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
Storage temp: 2 – 8 °C
References:
Purification, characterization, and immunolocalization of hydroxycinnamoyl-CoA: tyramine N-(hydroxycinnamoyl)transferase from opium poppy. Planta. (1999) 209: 33-44.
A new method for purification of human plasma retinol-binding protein and transthyretin. Biotechnol Appl Biochem. (2003) 38: 19-24.
Triazine dyes as inhibitors and affinity ligands of glycosyltransferases. Glycoconj J. (1999) 16: 719-23.
Well defined dye adsorbents for protein purification. J Mol Recognit. (1996) 9: 728-32.
Purification of human cholesteryl ester transfer protein by affinity chromatography on immobilized triazine dyes. Protein Expr Purif. (1994) 5: 285-90.
Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation. J Chromatogr. (1992) 584: 93-9.
Purification and characterization of vitamin B6-phosphate phosphatase from human erythrocytes. J Biol Chem. (1992) 267: 15978-83.
Dye-affinity purification of transthyretin from an unexploited by-product of human plasma chromatographic fractionation. J Chromatogr. (1992) 576: 87-93.
Identification and purification of a human Sertoli cell-secreted protein (hSCSP-80) stimulating Leydig cell steroid biosynthesis. J Clin Endocrinol Metab. (1991) 72: 1332-9.
Oestradiol-17 alpha dehydrogenase from chicken liver. Biochem J. (1984) 222: 761-8.
The interaction of yeast hexokinase with Procion Green H-4G. Biochem J. (1981) 197: 203-11.
Related products:
Red-Separopore® 4B-CL (SKU # 20181095)
Blue-Separopore® 4B-CL (SKU # 20181096)
Reactive Blue 4-Separopore® 4B-CL (SKU #20181097)
Green-Separopore® 4B-CL (SKU # 20181099)
Brown-Separopore® 4B-CL (SKU # 20181100)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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